Izumi Maria, Hano Kazuki, Kato Taiki, Hirata Akihiro, Thaw Paing, Seki Azusa, Tanaka Miyako, Ochi Kozue, Suganami Takayoshi, Kitagawa Hitoshi, Takasu Masaki
Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, Gifu, Japan.
Gifu University Institute for Advanced Study, Gifu University, Gifu, Japan.
J Vet Med Sci. 2025 Oct 1;87(10):1122-1127. doi: 10.1292/jvms.25-0145. Epub 2025 Aug 19.
We aimed to establish a streptozotocin-induced type 1 diabetes mellitus (DM) model in Microminipigs. Initially, a dose of 150 mg/kg streptozotocin was tested, and pigs exhibited characteristic triphasic patterns in glycemic responses: phase 1 (transient hyperglycemia), phase 2 (hypoglycemia), and phase 3 (sustained hyperglycemia). Blood glucose concentrations showed a transient increase in phase 1, and then dropped sharply in phase 2. Based on these findings, we conducted a follow-up study using a reduced streptozotocin dose of 125 mg/kg. This group exhibited glucose dynamics similar to those observed in the 150 mg/kg group, showing glucose reductions and mild lethargy. In phase 3, both groups developed sustained hyperglycemia with negligible insulin secretion, confirming the successful establishment of a type 1 DM model.
我们旨在建立链脲佐菌素诱导的小型猪1型糖尿病（DM）模型。最初，测试了150 mg/kg链脲佐菌素的剂量，猪在血糖反应中表现出典型的三相模式：第1阶段（短暂高血糖）、第2阶段（低血糖）和第3阶段（持续性高血糖）。血糖浓度在第1阶段短暂升高，然后在第2阶段急剧下降。基于这些发现，我们使用降低至125 mg/kg的链脲佐菌素剂量进行了后续研究。该组表现出与150 mg/kg组相似的葡萄糖动态变化，出现血糖降低和轻度嗜睡。在第3阶段，两组均出现持续性高血糖且胰岛素分泌可忽略不计，证实成功建立了1型糖尿病模型。
