Roth J A, Kaeberle M L
Am J Vet Res. 1985 Dec;46(12):2434-6.
Ascorbic acid (20 mg/kg of body weight) administered subcutaneously to otherwise nontreated cattle resulted in enhancement of neutrophil oxidative metabolism and capability of neutrophils to mediate antibody-dependent cell-mediated cytotoxicity (ADCC). Random migration, bacterial ingestion, and iodination by neutrophils was unaffected. Three dosage levels of ascorbic acid (10 mg/kg, 20 mg/kg, and 40 mg/kg) were examined for their effects on neutrophil function in cattle treated with dexamethasone (0.04 mg/kg). Dexamethasone administration caused an enhancement of neutrophil random migration and a suppression of neutrophil oxidative metabolism, iodination, and ADCC. None of the dosage levels of ascorbic acid had an effect on the alterations in the WBC count induced by dexamethasone. The ascorbic acid did tend to reverse the effects of dexamethasone on neutrophil random migration, oxidative metabolism, and ADCC in a dose-dependent manner, with the lowest dose having no discernible effect. Ascorbic acid administration also tended to enhance Staphylococcus aureus ingestion by bovine neutrophils. These results indicate that ascorbic acid should be further investigated for its potential to reduce the susceptibility of stressed or glucocorticoid-treated cattle to infective processes.
对未经其他处理的牛皮下注射抗坏血酸（20毫克/千克体重），可增强中性粒细胞的氧化代谢以及中性粒细胞介导抗体依赖性细胞介导的细胞毒性（ADCC）的能力。中性粒细胞的随机迁移、细菌吞噬和碘化作用不受影响。研究了三种剂量水平的抗坏血酸（10毫克/千克、20毫克/千克和40毫克/千克）对用 dexamethasone（0.04毫克/千克）处理的牛中性粒细胞功能的影响。给予dexamethasone会增强中性粒细胞的随机迁移，并抑制中性粒细胞的氧化代谢、碘化作用和ADCC。抗坏血酸的任何剂量水平均未对dexamethasone诱导的白细胞计数变化产生影响。抗坏血酸确实倾向于以剂量依赖性方式逆转dexamethasone对中性粒细胞随机迁移、氧化代谢和ADCC的影响，最低剂量没有明显效果。给予抗坏血酸还倾向于增强牛中性粒细胞对金黄色葡萄球菌的吞噬作用。这些结果表明，应进一步研究抗坏血酸在降低应激或糖皮质激素处理的牛对感染过程易感性方面的潜力。
