Han Xiaoyu, Cui Min, Ma Shuangxin, Gao Xuemei, Tang Liping, Li Bin
Academician Workstation, Jiangxi University of Chinese Medicine, Nanchang, 330004, China.
State Key Laboratory of Cancer Biology, Biotechnology Center, School of Pharmacy, Air Force Medical University of PLA, Xi'an, 710032, China.
BMC Plant Biol. 2025 Aug 20;25(1):1101. doi: 10.1186/s12870-025-07144-y.
The pleiotropic drug resistance (PDR) proteins, which are full-sized ABCG transporters belonging to the ATP-binding cassette (ABC) family, play an indispensable role in regulating various physiological and developmental processes of plants. However, the PDR transporters and their functional roles in Artemisia argyi, a significant traditional medicinal plant in Asia, remain poorly understood.
In this study, a total of 68 PDR genes, distributed across 17 chromosomes, were identified in the genome of Artemisia argyi and classified into five distinct subgroups based on phylogenetic analysis and domain types. The evolutionary characteristics investigation of the AarPDR gene family demonstrated that the 9 AarPDR genes had a high conservation between the genomes of Artemisia argyi and Arabidopsis during the evolution of dicots. Cis-element analysis revealed that AarPDRs might respond to diverse environmental stresses and multiple plant hormones. Furthermore, the expression analysis in different tissues and developmental stages of leaves indicated that various AarPDRs displayed distinct expression profiles within or among subfamilies, and the AarPDRs expression could be easily induced by the light conditions. Besides, the AarPDR family proteins had a potential function in metabolite transportation.
The AarPDRs were characterized by integrating genome analysis, transcriptome, and metabolite profiling. The results can provide useful clues for future studies aimed at the functional characterization of AarPDR genes.
多药耐药性（PDR）蛋白是属于ATP结合盒（ABC）家族的全尺寸ABCG转运蛋白，在调节植物的各种生理和发育过程中发挥着不可或缺的作用。然而，在亚洲一种重要的传统药用植物艾蒿中，PDR转运蛋白及其功能作用仍知之甚少。
在本研究中，在艾蒿基因组中鉴定出总共68个PDR基因，分布在17条染色体上，并根据系统发育分析和结构域类型分为五个不同的亚组。对AarPDR基因家族的进化特征研究表明，在双子叶植物进化过程中，9个AarPDR基因在艾蒿和拟南芥基因组之间具有高度保守性。顺式作用元件分析表明，AarPDRs可能对多种环境胁迫和多种植物激素作出反应。此外，对叶片不同组织和发育阶段的表达分析表明，不同的AarPDRs在亚家族内或亚家族间表现出不同的表达谱，并且AarPDRs的表达很容易受到光照条件的诱导。此外，AarPDR家族蛋白在代谢物运输中具有潜在功能。
通过整合基因组分析、转录组和代谢物谱对AarPDRs进行了表征。这些结果可为今后旨在对AarPDR基因进行功能表征的研究提供有用线索。
