Milsted A, Cohen W D, Lampen N
J Cell Sci. 1977 Feb;23:43-55. doi: 10.1242/jcs.23.1.43.
Mitotic spindles have been isolated from the blastema stage of Drosophila melanogaster embryos using modified tubulin-polymerizing medium. 'Clean' spindles, relatively free of contaminating cytoplasmic material, are obtained. Under phase contrast, mitotic stages appear remarkably similar to those seen in situ, as reported in early literature. This preservation of morphological integrity, coupled with relative structural simplicity due to low chromosome number (2n = 8), makes these spindles ideal subjects for study. Use of the scanning electron microscope provides excellent visulization of their general structural organization, changes in whole spindle structure during the course of mitosis, and higher resolution viewing of surface detail than is permitted with light microscopy.
使用改良的微管蛋白聚合培养基，从黑腹果蝇胚胎的芽基阶段分离出有丝分裂纺锤体。获得了相对不含细胞质污染物的“纯净”纺锤体。在相差显微镜下，有丝分裂阶段与早期文献报道的原位观察结果非常相似。这种形态完整性的保留，加上由于染色体数量少（2n = 8）而相对结构简单，使得这些纺锤体成为理想的研究对象。扫描电子显微镜的使用能够很好地观察到它们的总体结构组织、有丝分裂过程中整个纺锤体结构的变化，并且能比光学显微镜提供更高分辨率的表面细节观察。
