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分枝杆菌在-70℃保存：关键鉴别特征的持久性

Preservation of mycobacteria st -70 degrees C: persistence of key differential features.

作者信息

Kubica G P, Gontijo-Filho P P, Kim T

出版信息

J Clin Microbiol. 1977 Aug;6(2):149-53. doi: 10.1128/jcm.6.2.149-153.1977.

DOI:10.1128/jcm.6.2.149-153.1977
PMID:408373
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC274723/
Abstract

The advantages of long-term preservation of suspensions of mycobacteria by storage at -70 degrees C established in earlier studies are reinforced by present evidence that freezer storage does not alter key taxonomic features used to identify mycobacteria. Occasional discrepancies in biochemical test characteristics of mycobacteria that have been stored in the freezer and reconstituted at 37 degrees C reflect only sluggish metabolic activity, which is restored to normal on repeat testing.

摘要

早期研究表明，将分枝杆菌悬液保存在-70℃进行长期保存具有诸多优势，而目前的证据进一步强化了这一点，即冷冻保存不会改变用于鉴定分枝杆菌的关键分类特征。保存在冰箱中并在37℃复溶的分枝杆菌，其生化测试特征偶尔出现的差异仅反映出代谢活性迟缓，重复测试时该活性会恢复正常。

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本文引用的文献

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Differential identification of Mycobacteria. V. The tellurite reduction test.分枝杆菌的鉴别。五、亚碲酸盐还原试验。
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Mycobacterium intracellulare. Maintenance of pathogenicity in relationship to lyophilization and colony form.胞内分枝杆菌。与冻干和菌落形态相关的致病性维持。
Scand J Respir Dis. 1968;49(2):153-62.
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A co-operative numerical analysis of rapidly growing mycobacteria.快速生长分枝杆菌的合作数值分析。
J Gen Microbiol. 1972 Nov;73(1):55-70. doi: 10.1099/00221287-73-1-55.
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Long-term preservation and storage of mycobacteria.分枝杆菌的长期保存与储存
Appl Microbiol. 1972 Sep;24(3):311-7. doi: 10.1128/am.24.3.311-317.1972.
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Comparison of two commercial formulations of the MacConkey agar test for mycobacteria.用于分枝杆菌的麦康凯琼脂试验的两种商业制剂的比较。
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Differential identification of mycobacteria. VII. Key features for identification of clinically significant mycobacteria.分枝杆菌的鉴别诊断。VII. 临床重要分枝杆菌鉴定的关键特征。
Am Rev Respir Dis. 1973 Jan;107(1):9-21. doi: 10.1164/arrd.1973.107.1.9.
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Drug resistance in the Swedish/Norwegian BCG strain.瑞典/挪威卡介苗菌株中的耐药性。
Bull World Health Organ. 1972;46(4):503-7.
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