Kubica G P, Vitvitsky J
Appl Microbiol. 1974 May;27(5):917-9. doi: 10.1128/am.27.5.917-919.1974.
Recent evaluations of the MacConkey agar test for differentiation of rapidly growing mycobacteria have revealed that certain strains of Mycobacterium fortuitum and M. chelonei that were expected to grow on MacConkey agar failed to do so. Investigation of two formulations of MacConkey agar showed that these two species grew better on the medium when the crystal violet dye was omitted. Several possible reasons for this difficulty are discussed. It is recommended that clinical laboratories engaged in differential identification of mycobacteria utilize commercial MacConkey agar without crystal violet when testing rapidly growing species of this genus.
最近对用于区分快速生长分枝杆菌的麦康凯琼脂试验的评估表明，某些预计能在麦康凯琼脂上生长的偶然分枝杆菌和龟分枝杆菌菌株未能生长。对两种麦康凯琼脂配方的研究表明，当省略结晶紫染料时，这两个菌种在该培养基上生长得更好。讨论了造成这一困难的几个可能原因。建议从事分枝杆菌鉴别诊断的临床实验室在检测该属快速生长菌种时，使用不含结晶紫的商用麦康凯琼脂。
