冻干猴红细胞在麻疹病毒血凝及血凝抑制试验中的应用。

Application of freeze-dried monkey erythrocytes to measles viral hemagglutination and hemagglutination-inhibition tests.

作者信息

Iwasa S, Yoshida I, Ito H, Hori M

出版信息

J Clin Microbiol. 1977 Aug;6(2):176-8. doi: 10.1128/jcm.6.2.176-178.1977.

DOI:10.1128/jcm.6.2.176-178.1977

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC274729/

Abstract

Erthrocytes collected from monkey species including grivet, rhesus, and cynomolgus monkeys were stabilized by fixation with glutaraldehyde of a low concentration and were freeze-dried in vacuo. These freeze-dried erythrocytes were compared with fresh erythrocytes for measles viral hemagglutination and hemagglutination inhibition and could be used in both tests instead of fresh erythrocytes. They maintained their initial appearance and sensitivity to measles viral hemagglutinins after storage at 4 degrees C for 4 months more.

摘要

采集自包括埃及猿猴、恒河猴和食蟹猴等猴类物种的红细胞，用低浓度戊二醛固定使其稳定，然后真空冷冻干燥。将这些冷冻干燥的红细胞与新鲜红细胞进行麻疹病毒血凝和血凝抑制试验比较，结果表明它们可替代新鲜红细胞用于这两种试验。在4℃保存4个月以上后，它们仍保持初始外观以及对麻疹病毒血凝素的敏感性。

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本文引用的文献

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Studies on measles viral hemagglutination.麻疹病毒血凝作用的研究。

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A hemagglutination-inhibition technique for typing adenoviruses.一种用于腺病毒分型的血凝抑制技术。

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