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用于检测巨细胞病毒抗体的间接血凝试验的冻干红细胞。

Freeze-dried erythrocytes for an indirect hemagglutination test for detection of cytomegalovirus antibodies.

作者信息

Cabau N, Crainic R, Duros C, Denoyel G, Gaspar A, Bronnert C, Boué A, Horodniceanu F

出版信息

J Clin Microbiol. 1981 Jun;13(6):1026-30. doi: 10.1128/jcm.13.6.1026-1030.1981.

DOI:10.1128/jcm.13.6.1026-1030.1981
PMID:6265494
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC273943/
Abstract

An indirect hemagglutination test with lyophilized, fixed, tanned, and cytomegalovirus (CMV)-sensitized sheep erythrocytes for the detection of CMV antibodies is reported. To avoid nonspecific hemagglutination, cells were fixed with glutaraldehyde or Formalin directly in whole blood. The lyophilized, CMV-sensitized erythrocytes obtained by this technique were stable up to 9 months at 37 degrees C and retained the same reactivity at fresh, CMV-sensitized cells. Indirect hemagglutination performed with lyophilized, sensitized cells was highly efficient in detecting CMV-antibodies as compared with complement fixation and enzyme immunoassay.

摘要

本文报道了一种用冻干、固定、鞣酸处理并致敏巨细胞病毒（CMV）的绵羊红细胞进行间接血凝试验以检测CMV抗体的方法。为避免非特异性血凝，细胞直接在全血中用戊二醛或福尔马林固定。通过该技术获得的冻干、CMV致敏红细胞在37℃下可稳定保存9个月，并且与新鲜的CMV致敏细胞具有相同的反应性。与补体结合试验和酶免疫测定相比，用冻干致敏细胞进行的间接血凝试验在检测CMV抗体方面效率很高。

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引用本文的文献

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Immunity to human cytomegalovirus measured and compared by complement fixation, indirect fluorescent-antibody, indirect hemagglutination, and enzyme-linked immunosorbent assays.通过补体结合试验、间接荧光抗体试验、间接血凝试验和酶联免疫吸附测定法检测并比较人巨细胞病毒的免疫力。
J Clin Microbiol. 1984 Feb;19(2):147-52. doi: 10.1128/jcm.19.2.147-152.1984.

本文引用的文献

1
Assessment of human cytomegalovirus antibody detection techniques.人巨细胞病毒抗体检测技术评估
Arch Virol. 1980;64(4):287-301. doi: 10.1007/BF01320614.
2
Enzyme immunoassay for measurement of antibodies to herpes simplex virus infection: comparison with complement fixation, immunofluorescent-antibody, and neutralization techniques.用于检测单纯疱疹病毒感染抗体的酶免疫测定法：与补体结合法、免疫荧光抗体法及中和技术的比较
J Clin Microbiol. 1980 Feb;11(2):114-9. doi: 10.1128/jcm.11.2.114-119.1980.
3
Micro indirect hemagglutination test for Cytomegalovirus.巨细胞病毒微量间接血凝试验
Appl Microbiol. 1971 Jan;21(1):104-7. doi: 10.1128/am.21.1.104-107.1971.
4
Improved indirect hemagglutination test for cytomegalovirus using human O erythrocytes in lysine.使用赖氨酸处理的人O型红细胞改进巨细胞病毒间接血凝试验。
J Clin Microbiol. 1979 Jul;10(1):64-8. doi: 10.1128/jcm.10.1.64-68.1979.
5
Comparison of occurrence of antibodies to human cytomegalovirus as demonstrated by immunofluorescence and indirect hemagglutination techniques.通过免疫荧光和间接血凝技术对人巨细胞病毒抗体出现情况的比较。
J Clin Microbiol. 1979 Jan;9(1):149-51. doi: 10.1128/jcm.9.1.149-151.1979.
6
[Indirect hemagglutination test for detection of antibodies to cytomegalovirus in blood collected on blotting paper (author's transl)].用间接血凝试验检测滤纸上采集血液中巨细胞病毒抗体（作者译）
Pathol Biol (Paris). 1976 Oct;24(8):575-9.

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