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Reference gene identification and evaluation for accurate qPCR normalization in Phenacoccus manihoti Matile-Ferrero (Hemiptera: Pseudococcidae).

作者信息

Rao A Sunny, Reddy K V Nagarjuna, Ramasamy Gandhi Gracy, Reddy N Veeramanikanta, Negi Nikita, Rana Dhanendra Kumar, Sampathkumar M, Thiruvengadam Venkatesan, Sushil Satya N

机构信息

Division of Genomic Resources, ICAR-National Bureau of Agricultural Insect Resources, Bangalore, 560024, Karnataka, India.

Department of Entomology, Indira Gandhi Krishi Vishwavidyalaya, Raipur, 492012, Chattisgarh, India.

出版信息

Mol Biol Rep. 2025 Aug 21;52(1):838. doi: 10.1007/s11033-025-10922-4.

DOI:10.1007/s11033-025-10922-4
PMID:40839229
Abstract
摘要

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本文引用的文献

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RNAi silencing CHS1 gene shortens the mortality time of Plutella xylostella feeding Bt-transgenic Brassica napus.RNAi 沉默 CHS1 基因缩短了小菜蛾取食转 Bt 甘蓝的死亡时间。
Pest Manag Sci. 2024 Jun;80(6):2610-2618. doi: 10.1002/ps.7968. Epub 2024 Jan 22.
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Fusion dsRNA in targeting salivary protein genes enhance the RNAi-based aphid control.
靶向唾液蛋白基因的融合双链RNA增强基于RNA干扰的蚜虫防治效果。
Pestic Biochem Physiol. 2023 Dec;197:105645. doi: 10.1016/j.pestbp.2023.105645. Epub 2023 Oct 11.
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Selection and validation of reference genes for quantifying gene expression in the Gill's mealybug.用于定量测定吉尔粉蚧基因表达的内参基因的筛选与验证
J Econ Entomol. 2023 Dec 11;116(6):2166-2172. doi: 10.1093/jee/toad179.
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The most prominent modulated Annexins during parasitic infections.寄生感染过程中最主要的调节型 Annexin。
Acta Trop. 2023 Jul;243:106942. doi: 10.1016/j.actatropica.2023.106942. Epub 2023 May 10.
6
Reference genes selection for expression studies in Maconellicoccus hirsutus (Green) (Pseudococcidae: Hemiptera) under specific experimental conditions.在特定实验条件下选择研究麦圆叶爪绵蚧（Green）（粉蚧科：半翅目）表达的参考基因。
Mol Biol Rep. 2023 Feb;50(2):1221-1230. doi: 10.1007/s11033-022-08120-7. Epub 2022 Nov 27.
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Selection and Validation of Reference Genes for Quantitative Real-Time PCR Normalization in (Lepidoptera: Noctuidae) Under Different Conditions.不同条件下（鳞翅目：夜蛾科）定量实时PCR标准化参考基因的筛选与验证
Front Physiol. 2022 Feb 22;13:842195. doi: 10.3389/fphys.2022.842195. eCollection 2022.
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