Mackiewicz S, Wiktorowicz K, Mackiewicz A
Arch Immunol Ther Exp (Warsz). 1985;33(4):603-8.
The effect of CRP purified from ascites fluid originated from cancer patients on human peripheral blood lymphocytes was investigated. CRP was purified using 4-step procedure including absorption on Sepharose 4B, phosphocholine-Sepharose, DE-52 ion exchange chromatography and gel filtration on Sephacryl S-200. CRP was able either to inhibit or to enhance PHA-induced lymphocyte proliferation in vitro. The effects of added CRP depended on the time of its addition, dose of CRP and lymphocyte donor.
研究了从癌症患者腹水中纯化的CRP对人外周血淋巴细胞的影响。CRP采用四步程序进行纯化，包括在琼脂糖4B、磷酸胆碱-琼脂糖上吸附、DE-52离子交换色谱以及在Sephacryl S-200上进行凝胶过滤。CRP在体外既能抑制也能增强PHA诱导的淋巴细胞增殖。添加CRP的效果取决于其添加时间、CRP剂量和淋巴细胞供体。
