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Effects of temperature on actin polymerized by Ca2+. Direct evidence of fragmentation.

作者信息

Grazi E, Trombetta G

出版信息

Biochem J. 1985 Nov 15;232(1):297-300. doi: 10.1042/bj2320297.

DOI:10.1042/bj2320297
PMID:4084236
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1152875/
Abstract

When the temperature is lowered from 20 to 4 degrees C, the specific viscosity of actin polymerized in the presence of either 4 mM-CaCl2 or 2 mM-MgCl2, but not of actin polymerized in the presence of 90 mM-KCl, is decreased by 50% in the absence of free ATP. Addition of ATP restores the viscosity of the actin polymerized by Mg2+, but not that of actin polymerized by Ca2+, to the original value. The effect of temperature on actin polymerized in the presence of Ca2+ is due to (a) polymer-into-monomer conversion, (b) latero-lateral aggregation of filaments, and (c) fragmentation of the filaments. Fragmentation, as demonstrated by fractional centrifugation and electron microscopy, was the most important of these.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4686/1152875/d8c41a07fed1/biochemj00291-0283-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4686/1152875/d8c41a07fed1/biochemj00291-0283-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4686/1152875/d8c41a07fed1/biochemj00291-0283-a.jpg

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本文引用的文献

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肌动蛋白丝的断裂
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