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Kinetic heterogeneity of F-actin polymers. Further evidence that the elongation reaction may occur through condensation of the actin filaments with small aggregates.

作者信息

Grazi E, Magri E

机构信息

Istituto di Chimica Biologica, Università di Ferrara, Italy.

出版信息

Biochem J. 1987 Dec 15;248(3):721-5. doi: 10.1042/bj2480721.

DOI:10.1042/bj2480721
PMID:3435480
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1148609/
Abstract

We have shown that F-actin, polymerized in 50 mM-KCl at 20 degrees C and pH 8.0, can be resolved by centrifugation into two polymer populations, which differ morphologically as well as kinetically. The first population represents about 10% of the overall polymer and is composed of small amorphous aggregates. It rapidly exchanges the bound nucleotide with free ATP in the medium, either directly or through the monomers. The second population is composed of long actin filaments. These are labelled by free ATP in the medium only through condensation with labelled small amorphous aggregates.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1885/1148609/10386cd99615/biochemj00241-0104-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1885/1148609/10386cd99615/biochemj00241-0104-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1885/1148609/10386cd99615/biochemj00241-0104-a.jpg

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引用本文的文献

1
An alternative pathway of actin filament elongation. The condensation of small oligomers.肌动蛋白丝伸长的另一种途径。小寡聚物的凝聚。
J Muscle Res Cell Motil. 1989 Aug;10(4):275-9. doi: 10.1007/BF01758423.

本文引用的文献

1
F-ACTIN IS A RIGHT-HANDED HELIX.F-肌动蛋白是一种右手螺旋结构。
J Mol Biol. 1965 May;12:302-3. doi: 10.1016/s0022-2836(65)80306-0.
2
Identification of a factor in conventional muscle actin preparations which inhibits actin filament self-association.鉴定传统肌肉肌动蛋白制剂中一种抑制肌动蛋白丝自组装的因子。
Biochem Biophys Res Commun. 1980 Sep 16;96(1):18-27. doi: 10.1016/0006-291x(80)91175-4.
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Preparation of isolated thick and thin filaments.
Methods Enzymol. 1982;85 Pt B:277-84. doi: 10.1016/0076-6879(82)85026-x.
4
Characterization of the ATP-G-actin aggregates formed at low potassium chloride concentration.在低钾氯化物浓度下形成的ATP-G-肌动蛋白聚集体的特性分析。
Biochem J. 1984 Apr 1;219(1):273-6. doi: 10.1042/bj2190273.
5
Polymerization of actin and actin-like systems: evaluation of the time course of polymerization in relation to the mechanism.肌动蛋白及肌动蛋白样系统的聚合作用：聚合作用时间进程与机制的评估
Biochemistry. 1983 Dec 6;22(25):5836-43. doi: 10.1021/bi00294a023.
6
The polymerization of actin. A study of the nucleation reaction.肌动蛋白的聚合作用。成核反应的研究。
Biochem J. 1983 Sep 1;213(3):727-32. doi: 10.1042/bj2130727.
7
54Mn2+ as a tracer of the polymerization of actin. Intermediate oligomers condense to give F-actin.54Mn2+作为肌动蛋白聚合的示踪剂。中间寡聚体凝聚形成F-肌动蛋白。
Biochem J. 1984 Nov 1;223(3):571-6. doi: 10.1042/bj2230571.
8
The regulation of rabbit skeletal muscle contraction. I. Biochemical studies of the interaction of the tropomyosin-troponin complex with actin and the proteolytic fragments of myosin.兔骨骼肌收缩的调节。I. 原肌球蛋白-肌钙蛋白复合物与肌动蛋白及肌球蛋白蛋白水解片段相互作用的生化研究。
J Biol Chem. 1971 Aug 10;246(15):4866-71.
9
Electron microscope observations on actomyosin and actin preparations from Physarum polycephalum, and on their interaction with heavy meromyosin subfragment I from muscle myosin.对多头绒泡菌的肌动球蛋白和肌动蛋白制剂，以及它们与肌肉肌球蛋白的重酶解肌球蛋白亚片段I相互作用的电子显微镜观察。
J Mol Biol. 1970 May 28;50(1):83-90. doi: 10.1016/0022-2836(70)90105-1.
10
Bovine aorta actin. Development of an improved purification procedure and comparison of polymerization properties with actins from other types of muscle.牛主动脉肌动蛋白。一种改进的纯化方法的开发以及与其他类型肌肉的肌动蛋白聚合特性的比较。
Biochim Biophys Acta. 1985 Mar 22;828(1):13-21. doi: 10.1016/0167-4838(85)90003-2.