Wang Fangyuan, Jia Jinrong, Gu Wei
Institute of Plant Protection, Heilongjiang Academy of Agricultural Sciences, Harbin, China.
Front Microbiol. 2025 Aug 6;16:1652141. doi: 10.3389/fmicb.2025.1652141. eCollection 2025.
The continuous and extensive use of pesticides has negative impacts on the environment and human health. Microbial remediation is an eco-friendly and economically efficient technology, which is of great significance. In this investigation, the degradation of the herbicide Diquat by yeast Wyslmt was studied in medium under different conditions. The degradation rate of Diquat showed a pattern of first increasing and then decreasing with the increase of the inoculation amount of Wyslmt, temperature, pH and the initial concentration of Diquat. The biodegradation transformation products (BTPs) formed by the microbial degradation of Diquat in the culture medium solution were isolated and identified by ultra-high-performance liquid chromatography coupled with time-of-fight mass spectrometry (UPLC-QTOF-MS). Based on the mass spectrometry information, three main transformation products were determined. The calculation of components, the comparison of structural analogues, and the existing literature are all helpful for the determination of the structure. The main pathway of microbial degradation was C-C bond broken, hydroxylation and demethylation. These results lay the foundation for further environmental risk assessment and provide a reference for the bioremediation evaluation of bipyridine herbicides.
农药的持续广泛使用对环境和人类健康产生负面影响。微生物修复是一种环保且经济高效的技术，具有重要意义。在本研究中，研究了酵母Wyslmt在不同条件下对除草剂敌草快在培养基中的降解情况。敌草快的降解率随着Wyslmt接种量、温度、pH值和敌草快初始浓度的增加呈现先升高后降低的趋势。通过超高效液相色谱-飞行时间质谱联用仪（UPLC-QTOF-MS）对培养基溶液中敌草快微生物降解形成的生物降解转化产物（BTPs）进行分离和鉴定。基于质谱信息，确定了三种主要转化产物。通过计算组分、比较结构类似物以及参考现有文献，均有助于结构的确定。微生物降解的主要途径是碳-碳键断裂、羟基化和去甲基化。这些结果为进一步的环境风险评估奠定了基础，并为联吡啶类除草剂的生物修复评价提供了参考。
