Yelleti Geethika, Aroor Annayya Rao, Shenoy Revathi Panduranga, Karmakar Abhibroto, Rao Ashwini, Lewis Cheryl Rhea, Asok Arjun, Maripini Nihaal, Biswas Monalisa, Varier Veena, Roy Arpita, Suryakanth Varashree Bolar
Department of Biochemistry, Kasturba Medical college, Manipal, Manipal Academy of Higher Education, Manipal, Karnataka, India, 576104.
Department of Medicine, University of Missouri, Columbia Missouri MO, USA 65212.
Rep Biochem Mol Biol. 2025 Jan;13(4):495-506. doi: 10.61186/rbmb.13.4.495.
Preeclampsia (PE) is a hypertensive disorder in pregnancy affecting multiple organ systems. This study hypothesized that oxidative stress and inflammatory responses contribute to the pathogenesis of Preeclampsia, and that selenium and N-acetylcysteine (NAC) could mitigate these effects.
The study was initiated after approval from the Institutional Animal Ethics Committee. Twenty-four female Wistar rats were divided equally into four groups. Group I served as controls, while Groups II, III, and IV received Nω-Nitro-L-arginine methyl ester hydrochloride (L-NAME) to induce hypertension from day 10 to 20 of gestation. Additionally, Group III received selenium (240 μg/kg/day) and Group IV received NAC (160 mg/kg). On day 20, Blood Pressure (BP) monitoring and urine protein estimation were carried out to assess hypertension and proteinuria, while blood samples were collected to measure malondialdehyde (MDA) and interleukin-6 (IL-6) levels, as markers of oxidative stress and inflammation, respectively. Statistical analysis was performed using GraphPad Prism 10.2.
Selenium improved L-NAME-induced hypertension (Mean BP 107.63±5.22 mmHg vs 140.9±8.38 mmHg in disease control (DC) and proteinuria (65.5±4.09 vs 140.2±11.85 mg/day in DC) and significantly reduced the inflammatory response (IL-6 23.4±1.06 vs 50.63±3.35 pg/mL in DC) but had little effect on oxidative stress (MDA 0.21±0.02 vs 0.24±0.02 nmol/mL in DC). NAC did not lower BP (Mean BP 129.33±7.96 mmHg) but significantly reduced proteinuria (92.7±6.37mg/day), IL-6 levels (18.24±0.42 pg/mL), and oxidative stress (MDA 0.16±0.01 nmol/mL).
These findings suggest that selenium and NAC play distinct protective roles in the pathophysiology of preeclampsia, potentially offering synergistic effects for cardiovascular and kidney health in hypertensive pregnancies.
子痫前期（PE）是一种影响多个器官系统的妊娠期高血压疾病。本研究假设氧化应激和炎症反应参与子痫前期的发病机制，且硒和N-乙酰半胱氨酸（NAC）可减轻这些影响。
本研究经机构动物伦理委员会批准后启动。24只雌性Wistar大鼠平均分为四组。第一组作为对照组，而第二、三、四组在妊娠第10天至20天接受盐酸Nω-硝基-L-精氨酸甲酯（L-NAME）以诱导高血压。此外，第三组接受硒（240μg/kg/天），第四组接受NAC（160mg/kg）。在第20天，进行血压（BP）监测和尿蛋白评估以评估高血压和蛋白尿，同时采集血样以测量丙二醛（MDA）和白细胞介素-6（IL-6）水平，分别作为氧化应激和炎症反应的标志物。使用GraphPad Prism 10.2进行统计分析。
硒改善了L-NAME诱导的高血压（平均血压107.63±5.22mmHg，疾病对照组（DC）为140.9±8.38mmHg）和蛋白尿（65.5±4.09对DC组的140.2±11.85mg/天），并显著降低炎症反应（IL-6 23.4±1.06对DC组的50.63±3.35pg/mL），但对氧化应激影响不大（MDA 0.21±0.02对DC组的0.24±0.02nmol/mL）。NAC未降低血压（平均血压129.33±7.96mmHg），但显著降低了蛋白尿（92.7±6.37mg/天）、IL-6水平（18.24±0.42pg/mL）和氧化应激（MDA 0.16±0.01nmol/mL）。
这些发现表明，硒和NAC在子痫前期的病理生理学中发挥着不同的保护作用，可能为高血压妊娠中的心血管和肾脏健康提供协同效应。
