Karimi Samaneh, Koohpeyma Farhad, Khorsandi Layasadat, Panahi Armin, Rezaei-Tazangi Fatemeh, Fakhredini Fereshtesadat
Department of Anatomical Sciences, School of Medicine, Abadan University of Medical Sciences, Abadan, Iran.
Student Research Committee, Endocrinology and Metabolism Research Center, Shiraz University of Medical Science, Shiraz, Iran.
Rep Biochem Mol Biol. 2025 Jan;13(4):579-589. doi: 10.61186/rbmb.13.4.579.
Unilateral nephrectomy and renal ischemia-reperfusion injury (I/R) are causes of acute kidney injury that can cause renal dysfunction, decreased glomerular filtration rate, impaired homeostasis, and increased mortality. This study aimed to evaluate the combined effects of curcumin (Cur) and metformin (Met), both protective antioxidants, on renal tissue structure and Neutrophil gelatinase-associated lipocalin (Ngal) and Kidney injury molecule 1 (Kim-1) genes expression levels in rats undergoing unilateral nephrectomy and ischemia/reperfusion.
In this experimental study, 40 male rats were randomly divided into five groups. The animals underwent unilateral nephrectomy and ischemia/reperfusion and were then treated with curcumin and metformin or both for 14 days. Subsequently, histopathological and morphometric analyses were performed, and Ngal and Kim-1 gene expression levels were evaluated using real-time PCR.
Histopathological damage, expression levels of blood urea nitrogen (BUN), creatinine (Cr), blood biochemical parameters and oxidative stress and expression levels of Ngal and Kim-1 genes were significantly reduced in the patient group that received metformin and curcumin simultaneously. However, total antioxidant capacity (TAC) was increased in the patient group that received curcumin + metformin. Morphometric parameters also improved in this group.
The results showed that the combination therapy with curcumin and metformin effectively protected the kidneys against unilateral nephrectomy and I/R injury.
单侧肾切除术和肾缺血再灌注损伤（I/R）是急性肾损伤的病因，可导致肾功能障碍、肾小球滤过率降低、体内稳态受损及死亡率增加。本研究旨在评估姜黄素（Cur）和二甲双胍（Met）这两种具有保护作用的抗氧化剂联合使用对接受单侧肾切除术和缺血/再灌注的大鼠肾组织结构以及中性粒细胞明胶酶相关脂质运载蛋白（Ngal）和肾损伤分子1（Kim-1）基因表达水平的影响。
在本实验研究中，40只雄性大鼠被随机分为五组。动物接受单侧肾切除术和缺血/再灌注，然后用姜黄素和二甲双胍或两者同时处理14天。随后，进行组织病理学和形态计量学分析，并使用实时PCR评估Ngal和Kim-1基因表达水平。
同时接受二甲双胍和姜黄素的患者组的组织病理学损伤、血尿素氮（BUN）、肌酐（Cr）、血液生化参数和氧化应激的表达水平以及Ngal和Kim-1基因的表达水平均显著降低。然而，接受姜黄素+二甲双胍的患者组的总抗氧化能力（TAC）增加。该组的形态计量学参数也有所改善。
结果表明，姜黄素和二甲双胍联合治疗可有效保护肾脏免受单侧肾切除术和I/R损伤。
