de Melo-Ximenes Amanda Alves, Batista Romina, Corvalán Leonardo Carlos Jeronimo, Marques-Bonet Tomas, Kuderna Lukas, Farh Kyle, Rogers Jeffrey, Kaizer Mariane da Cruz, Boubli Jean Philippe, de Melo Fabiano Rodrigues, Nunes Rhewter, Telles Mariana Pires de Campos
Laboratório de Genética & Biodiversidade Instituto de Ciências Biológicas I, Universidade Federal de Goiás Goiânia Goiás Brazil.
Instituto Nacional de Pesquisas da Amazônia, INPA Manaus Amazonas Brazil.
Ecol Evol. 2025 Aug 19;15(8):e71356. doi: 10.1002/ece3.71356. eCollection 2025 Aug.
Genomic resources, such as draft genomes, are vital for biodiversity monitoring and conservation. For endangered species, they enable the development of tools like organellar genomes and molecular markers, which are crucial for population genetics. Advances in sequencing technologies now allow high-throughput genotyping with detailed amplicon sequences, enhancing genetic variation studies. The northern muriqui (), a critically endangered primate endemic to Brazil's Atlantic Forest, currently lacks both nuclear and mitochondrial genome data and species-specific microsatellite markers for population genetic studies. We assembled a 2.52 Gb draft genome for with 202,243 contigs (N50 = 29,134 bp), and BUSCO analyses indicated 52% completeness and 15.5% fragmented genes. The complete 16,635 bp mitochondrial genome retains the conserved mammalian structure with 22 tRNAs, 2 rRNAs, 13 CDS, and an origin of replication. Additionally, we designed 31 SSR primer pairs suitable for non-invasive sampling and genotyping, alongside two mtDNA and two sex-determination primers, configured into three multiplex PCR sets. These genomic resources, including the draft genome, complete mitochondrial genome, and microsatellite markers, provide essential tools for evolutionary analyses and the genetic monitoring of populations, supporting its conservation.
基因组资源，如基因组草图，对于生物多样性监测和保护至关重要。对于濒危物种而言，它们有助于开发细胞器基因组和分子标记等工具，这些对于群体遗传学至关重要。测序技术的进步现在允许进行具有详细扩增子序列的高通量基因分型，从而加强遗传变异研究。北方绒毛蛛猴是巴西大西洋森林特有的极度濒危灵长类动物，目前缺乏用于群体遗传学研究的核基因组和线粒体基因组数据以及物种特异性微卫星标记。我们为北方绒毛蛛猴组装了一个2.52Gb的基因组草图，有202,243个重叠群（N50 = 29,134bp），BUSCO分析表明其完整性为52%，基因片段化率为15.5%。完整的16,635bp线粒体基因组保留了保守的哺乳动物结构，有22个tRNA、2个rRNA、13个CDS和一个复制起点。此外，我们设计了31对适用于非侵入性采样和基因分型的SSR引物对，以及两对线粒体DNA引物和两对性别鉴定引物，配置成三个多重PCR组。这些基因组资源，包括基因组草图、完整的线粒体基因组和微卫星标记，为北方绒毛蛛猴种群的进化分析和遗传监测提供了重要工具，有助于其保护。
