Gandomkar Habibollah, Shekarabi Seyed Pezhman Hosseini, Abdolhay Hossein Ali, Nazari Sajad, Mehrgan Mehdi Shamsaie
Department of Fisheries, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Agricultural Research, Education and Extension Organization (AREEO), Iranian Fisheries Sciences Research Institute, Tehran, Iran.
Mol Biol Rep. 2021 Sep;48(9):6471-6480. doi: 10.1007/s11033-021-06653-x. Epub 2021 Aug 22.
The species Capoeta aculeata (Valenciennes, 1844) is one of the most important freshwater species endemic to Iran. However, the investigation of the population genetic structure of this species is limited by the low number of molecular markers currently described.
In this study, we implemented next generation sequencing technology to identify polymorphic microsatellite markers and investigate the population genetic structure of C. aculeata sampled from three geographical sites in Iran. We characterized and developed 36 novel polymorphic microsatellite markers and these loci were examined in 120 individuals from three populations occurring in the Zagros basin. The average number of alleles per locus varied from 1.7 to 16 (average = 7.89). The results showed that, the polymorphism information content (PIC) of these simple sequence repeat (SSR) loci varied from 0.254 to 0.888. The observed heterozygosity (H) per locus ranged from 0.170 to 0.881, while the expected heterozygosity (H) per locus was from 0.170 to 0.881. Among these SSR loci, 20 loci deviated significantly from the Hardy-Weinberg equilibrium after Bonferroni correction (p < 0.05).
These microsatellite markers could provide a valuable tool for future population and conservation genetics studies of C. aculeate and other closely related species.
阿氏突吻鱼（Capoeta aculeata，瓦朗谢讷，1844年）是伊朗特有的最重要的淡水物种之一。然而，目前该物种的分子标记数量较少，限制了对其种群遗传结构的研究。
在本研究中，我们采用下一代测序技术来鉴定多态性微卫星标记，并研究从伊朗三个地理区域采集的阿氏突吻鱼的种群遗传结构。我们鉴定并开发了36个新的多态性微卫星标记，并在来自扎格罗斯盆地三个种群的120个个体中对这些位点进行了检测。每个位点的平均等位基因数从1.7到16不等（平均 = 7.89）。结果表明，这些简单序列重复（SSR）位点的多态性信息含量（PIC）从0.254到0.888不等。每个位点的观察杂合度（H）范围为0.170至0.881，而每个位点的预期杂合度（H）为0.170至0.881。在这些SSR位点中，经Bonferroni校正后，有20个位点显著偏离哈迪-温伯格平衡（p < 0.05）。
这些微卫星标记可为未来阿氏突吻鱼及其他近缘物种的种群和保护遗传学研究提供有价值的工具。
