Helmeke Michelle Marie B, Haynie-Cion Rhianna L, Pratt Matthew R
Department of Chemistry, University of Southern California Los Angeles California 90089 USA
RSC Chem Biol. 2025 Jul 29. doi: 10.1039/d5cb00168d.
Metabolic oligosaccharide engineering (MOE) is a transformative technology, enabling the chemical labeling and subsequent analysis of glycans. Central to this method are monosaccharide analogs, termed metabolic chemical reporters (MCRs), that contain abiotic functional groups that can undergo an increasing number of bioorthogonal reactions. Typically, these abiotic groups were designed to be as small as possible, allowing them to be tolerated by metabolic enzymes and glycosyltransferases that transform MCRs into donor sugars and add them into glycans, respectively. This generality allows MCRs to be used by a variety of cells and tissues but can also be a limitation in their application to investigate glycosylation of specific cell-types in multicellular systems. Here, we review different methods that are beginning to transition MCRs into cell selective tools, with the potential to increase the already large impact these compounds have had on glycoscience.
代谢寡糖工程（MOE）是一项变革性技术，能够对聚糖进行化学标记并随后进行分析。该方法的核心是单糖类似物，称为代谢化学报告分子（MCR），其含有可进行越来越多生物正交反应的非生物官能团。通常，这些非生物基团设计得尽可能小，以使它们能够被代谢酶和糖基转移酶耐受，这些酶分别将MCR转化为供体糖并将它们添加到聚糖中。这种通用性使MCR能够被多种细胞和组织使用，但在将其应用于研究多细胞系统中特定细胞类型的糖基化时也可能成为一种限制。在这里，我们综述了不同的方法，这些方法开始将MCR转变为细胞选择性工具，有可能增加这些化合物对糖科学已经产生的巨大影响。
