Tapily El Hadj Hussein, Kouassi Kan Modeste, Kouassi Marius Konan, Seka John Steven S, Tiendrébéogo Fidèle, Pita Justin S
Unité de Formation et de Recherche Biosciences, Université Félix Houphouët-Boigny (UFHB), Abidjan 22 BP 582, Côte d'Ivoire.
The Central and West African Virus Epidemiology (WAVE) for Food Security Program, Pôle Scientifique et d'Innovation, Univesité Félix Houphouët-Boigny (UFHB), Bingerville 01 BP V34, Côte d'Ivoire.
BioTech (Basel). 2025 Aug 19;14(3):63. doi: 10.3390/biotech14030063.
Regenerating sweet potato from field-derived plant material requires careful management of several critical factors, including the effectiveness of the disinfectant, the age of the explant, and the genotype used. In this context, establishing a reliable aseptic protocol is essential for successful in vitro culture. This study aimed to assess the effects of two disinfectants (sodium hypochlorite and mercuric chloride), three sweet potato genotypes (Nakabo, Boyapleu, and Irene), and three explant ages (2, 3, and 4 weeks) on clean culture establishment and regeneration efficiency from nodal explants. The findings revealed that regeneration success is significantly influenced by the type and concentration of disinfectant, explant age, and genotype. Treatment with 10% sodium hypochlorite markedly reduced contamination, achieving clean culture and regeneration rates of 75.72 ± 3.36% and 86.83 ± 3.02%, respectively, regardless of explant age. In contrast, higher concentrations of mercuric chloride induced necrosis in the explants. The highest clean culture rate (93.82 ± 1.16%) was observed in 3-week-old explants, which also showed a regeneration rate of 54.93 ± 3.19%. Furthermore, the Boyapleu and Irene genotypes demonstrated good suitability for in vitro culture, whereas the Nakabo genotype performed poorly under the tested conditions.
利用田间来源的植物材料再生甘薯需要仔细管理几个关键因素，包括消毒剂的有效性、外植体的年龄和所用的基因型。在这种情况下，建立可靠的无菌方案对于成功进行离体培养至关重要。本研究旨在评估两种消毒剂（次氯酸钠和氯化汞）、三种甘薯基因型（中保、博亚普勒和艾琳）以及三种外植体年龄（2、3和4周）对从节段外植体建立无菌培养和再生效率的影响。研究结果表明，再生成功率受到消毒剂的类型和浓度、外植体年龄和基因型的显著影响。用10%次氯酸钠处理可显著减少污染，无论外植体年龄如何，无菌培养率和再生率分别达到75.72±3.36%和86.83±3.02%。相比之下，较高浓度的氯化汞会导致外植体坏死。在3周龄的外植体中观察到最高的无菌培养率（93.82±1.16%），其再生率也为54.93±3.19%。此外，博亚普勒和艾琳基因型表现出良好的离体培养适应性，而中保基因型在测试条件下表现较差。
