Matos-Sousa José Mário, Melo Wallacy Watson Pereira, Dos Santos Vinicius Ruan Neves, Pereira Cristian Dos Santos, Mendes Paulo Fernando Santos, Lemos Flávia Letícia Magalhães, Pinheiro João de Jesus Viana, Fernandes Luanna de Melo Pereira, Maia Cristiane do Socorro Ferraz, Chaves-Neto Antônio Hernandes, Lima Rafael Rodrigues
Laboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Pará, Augusto Corrêa Street, Guamá, Belém, 66075-110, Pará, Brazil.
Laboratory of Anatomopathology and Immunohistochemistry, School of Dentistry, Federal University of Pará, Belém, 66075-110, Pará, Brazil.
Naunyn Schmiedebergs Arch Pharmacol. 2025 Aug 22. doi: 10.1007/s00210-025-04491-3.
This study investigated the effects of high-intense ethanol consumption during abstinence periods on the biochemical and morphological profile of the salivary glands and saliva of rats. For this, 72 male rats, 35 days old, exposed to ethanol (20% w/v) in 4 cycles of binge drinking at a dose of 3 g/kg/day were used. The animals were evaluated 1 day after the last cycle, as well as 30 and 60 days of abstinence. The parotid and submandibular glands were collected for oxidative biochemical and morphological analysis while saliva for antioxidant and total protein analysis. Student's t-test was used for statistical comparisons of each analysis period (p = 0.05). In the parotid, when compared with the control group, an increase in reduced glutathione was observed in 60 days (p = 0.04), a decrease in total antioxidant capacity in 1 day (p = 0.007), 30 days (p = 0.006) and 60 days (p = 0.001) were observed, as well as an increase in lipid peroxidation (p = 0.009) and nitric oxide (p < 0.0001) in 60 days. Morphologically, there was a reduction in the area of acini in 1, 30, and 60 days (p < 0.05). In the submandibular, there was an increase in lipid peroxidation in 1 day (p = 0.0197) and nitric oxide in 1 and 30 days (p < 0.0001). Regarding morphological changes, a reduction in the area of acini and an increase in stroma were observed at 1 and 60 days (p < 0.0001). It was concluded that exposure to ethanol during adolescence generates prolonged biochemical and morphological changes in the salivary glands and the saliva.
本研究调查了禁戒期高强度乙醇摄入对大鼠唾液腺及唾液的生化和形态学特征的影响。为此，选用了72只35日龄雄性大鼠，使其以3 g/kg/天的剂量在4个暴饮周期中接触乙醇（20% w/v）。在最后一个周期后的第1天以及禁戒30天和60天时对动物进行评估。收集腮腺和颌下腺用于氧化生化和形态学分析，收集唾液用于抗氧化和总蛋白分析。采用学生t检验对每个分析期进行统计学比较（p = 0.05）。在腮腺中，与对照组相比，60天时还原型谷胱甘肽增加（p = 0.04），1天（p = 0.007）、30天（p = 0.006）和60天（p = 0.001）时总抗氧化能力降低，60天时脂质过氧化（p = 0.009）和一氧化氮（p < 0.0001）增加。形态学上，1天、30天和60天时腺泡面积减小（p < 0.05）。在颌下腺中，1天时脂质过氧化增加（p = 0.0197），1天和30天时一氧化氮增加（p < 0.0001）。关于形态学变化，1天和60天时观察到腺泡面积减小和间质增加（p < 0.0001）。得出的结论是，青春期接触乙醇会在唾液腺和唾液中产生长期的生化和形态学变化。
