Liu Bin, Jiang Hanlin, Zhao Tingrui, Ito Akira, Moriyama Hideki
Department of Rehabilitation Science, Graduate School of Health Sciences, Kobe University, Kobe, Japan.
Department of Motor Function Analysis, Human Health Sciences, Graduate School of Medicine, Kyoto, Japan.
Calcif Tissue Int. 2025 Aug 22;116(1):112. doi: 10.1007/s00223-025-01422-5.
Bone fractures, especially in the elderly, are increasing, posing challenges to healthcare systems. Traditional treatment often focuses merely on bone repair and overlooks the overall healing environment. Peripheral magnetic stimulation (PMS), a non-invasive method, shows promise for bone regeneration. In our murine femoral fracture model study, male C57BL6/J mice were divided into a control group and three PMS treatment groups (10, 50, and 100 Hz). After a fracture, the mice received 30-min daily PMS sessions. The pain was monitored weekly using the von Frey test. Micro-computed tomography (μCT), biomechanics, and histology evaluated bone healing. Our results have shown that 10 and 100 Hz PMS significantly reduced pain and promoted early callus formation by speeding up early mineralized callus. After 4 weeks, the 10 Hz PMS improved mechanical strength, and the 10 and 100 Hz PMS increased bone mineral density. Histology revealed more cartilage, new bone formation, and enhanced osteoblast activity. PMS also decreased fibrous tissue, indicating better bone remodeling. The staining results confirmed that PMS promoted early cartilage formation, endochondral ossification, and increased vascular density. These findings suggest that PMS at 10 and 100 Hz accelerates endochondral ossification, enhances bone formation, and improves biomechanical strength, demonstrating its potential application value in fracture treatment.
骨折，尤其是在老年人中，正日益增多，给医疗保健系统带来了挑战。传统治疗往往仅侧重于骨骼修复，而忽视了整体愈合环境。外周磁刺激（PMS）作为一种非侵入性方法，在骨再生方面显示出前景。在我们的小鼠股骨骨折模型研究中，将雄性C57BL6/J小鼠分为对照组和三个PMS治疗组（10、50和100赫兹）。骨折后，小鼠每天接受30分钟的PMS治疗。每周使用von Frey测试监测疼痛情况。通过微计算机断层扫描（μCT）、生物力学和组织学评估骨愈合情况。我们的结果表明，10赫兹和100赫兹的PMS通过加速早期矿化骨痂显著减轻疼痛并促进早期骨痂形成。4周后，10赫兹的PMS提高了机械强度，10赫兹和100赫兹的PMS增加了骨密度。组织学显示软骨更多、新骨形成以及成骨细胞活性增强。PMS还减少了纤维组织，表明骨重塑更好。染色结果证实PMS促进了早期软骨形成、软骨内成骨并增加了血管密度。这些发现表明，10赫兹和100赫兹的PMS加速软骨内成骨、增强骨形成并提高生物力学强度，证明了其在骨折治疗中的潜在应用价值。
