Delbarre A, Delepierre M, Igolen J, Le Pecq J B, Roques B P
Biochimie. 1985 Jul-Aug;67(7-8):823-8. doi: 10.1016/s0300-9084(85)80174-7.
The structure of the complexes formed between a 7H-pyridocarbazole dimer (ditercalinium) or the corresponding monomer and d[CpGpCpG] is analyzed in aqueous solution by 270 MHz 1H NMR. In both cases the strong upfield shifts observed on most aromatic resonances are assigned to the formation of intercalated complexes. Bisintercalation of the dimer in the tetranucleotide minihelix is then observed at pH 5.5. The observation of intermolecular negative NOEs induced to some drug resonances by irradiation of sugar protons confirms these conclusions. The orientation of the ligand in the intercalation site is discussed.
通过270 MHz的1H NMR对7H-吡啶并咔唑二聚体（双特卡林）或相应单体与d[CpGpCpG]在水溶液中形成的复合物结构进行了分析。在这两种情况下，大多数芳香族共振峰上观察到的强烈高场位移都归因于插入复合物的形成。然后在pH 5.5时观察到二聚体在四核苷酸小螺旋中的双插入。通过照射糖质子对某些药物共振诱导的分子间负NOE的观察证实了这些结论。讨论了配体在插入位点的取向。
