Renan M J
Biosci Rep. 1985 Sep;5(9):739-53. doi: 10.1007/BF01119872.
In this study, the sequences of several cellular genes (c-myc, c-fos, c-sis, c-mos, and the genes for urokinase, heat shock proteins, interleukin-2 and its receptor), thought to be controlled by negative regulatory factors, were examined. As a result of this comparison, multiple (and often clustered) copies of a 12 basepair (bp) element were identified in the flanking regions of these genes. Moreover, sequences with close homology to this 12 bp element were identified in specific control regions of some DNA and RNA tumor viruses. A consensus sequence (TTG nnn TTTTTT) was derived from an analysis of 111 of these elements. These sequence homologies have yielded a coherent first hypothesis, namely that this 12 bp element is the binding site of a transcriptional repressor protein.
在本研究中，对几个被认为受负调控因子控制的细胞基因（c-myc、c-fos、c-sis、c-mos以及尿激酶、热休克蛋白、白细胞介素-2及其受体的基因）序列进行了检测。通过这种比较，在这些基因的侧翼区域鉴定出了一个12碱基对（bp）元件的多个（且常常成簇）拷贝。此外，在一些DNA和RNA肿瘤病毒的特定控制区域中也鉴定出了与这个12 bp元件具有高度同源性的序列。通过对其中111个元件的分析得出了一个共有序列（TTG nnn TTTTTT）。这些序列同源性得出了一个连贯的初步假设，即这个12 bp元件是转录抑制蛋白的结合位点。
