Puymirat J, Faivre-Bauman A, Barret A, Loudes C, Tixier-Vidal A
Brain Res. 1985 Dec;355(2):315-7. doi: 10.1016/0165-3806(85)90058-6.
Serum-free cultures of mouse mesencephalic cells were used for studying the influence of triiodothyronine (T3) on the morphogenesis of dopaminergic neurons. Supplementation of culture medium with T3 (10(-9) M) did not modify the number, but increased the size of the perikarya without affecting the neurite density of mesencephalic dopamine (DA) neurons. Furthermore, T3 had no effect on the uptake of [3H]DA in these cultures. These findings, taken together with previous results obtained with hypothalamic cell cultures, show that T3 affects the morphogenesis of mesencephalic DA neurons in a different manner than that of hypothalamus and that the perikarya size on the one hand, and the neurite elongation on the other hand, seem to be independently regulated by T3.
小鼠中脑细胞的无血清培养用于研究三碘甲状腺原氨酸（T3）对多巴胺能神经元形态发生的影响。在培养基中添加T3（10^(-9) M）不会改变中脑多巴胺（DA）神经元的数量，但会增加其胞体大小，而不影响神经突密度。此外，T3对这些培养物中[3H]DA的摄取没有影响。这些发现与先前在下丘脑细胞培养中获得的结果一起表明，T3对中脑DA神经元形态发生的影响方式与下丘脑不同，并且胞体大小和神经突伸长似乎由T3独立调节。
