Zou Chengyi, Dai Tianfei, Liang Zhonghua, Li Mingyan, Meng Lina, Zhao Xianming, Li Nianzhen, Wei Qin, Abd Elhamid Mohamed A, Atif Amr M, Soaud Salma A, Wen Mengling, Yan Kuan, El-Sappah Ahmed H
Faculty of Agriculture, Forestry and Food Engineering, Yibin University, Yibin, China.
Sichuan Green Food Development Center, Chengdu, China.
Front Microbiol. 2025 Aug 7;16:1651997. doi: 10.3389/fmicb.2025.1651997. eCollection 2025.
Intercropping systems can significantly influence soil microbial communities, affecting plant health and soil nutrient cycling, which has better economic and ecological benefits than monoculture of tea.
This study investigated the impact of loquat-tea intercropping on the microbial community structure and functional gene composition in the rhizosphere soil of tea (). Using metagenomic sequencing, we analyzed rhizosphere soils from loquat-tea intercropping (PP_CS), tea monoculture (CS), and loquat monoculture (PP).
A total of 161 phyla, 269 classes, 485 orders, 1,000 families, 3,838 genera, and 27,624 species were annotated across samples. Dominant phyla included , , , and . The genera (4.20%) and (3.78%) were notably enriched in the intercropping system. The analysis of community differences showed that unclassified_c_acidobacteria was in pp_cs group, demonstrating the highest LDA score (4.4 score). Functional annotation via the Kyoto Encyclopedia of Genes and Genomes (KEGG) revealed that metabolic pathways were predominant across all treatments, with 36,111,608 reads assigned to metabolism. The comparative analysis at KEGG level 3 revealed that 289 constituted the most abundantly annotated functional category across all three groups. Redundancy analysis (RDA) showed strong correlations between key microbial genera (, ) and soil properties, including organic matter (OM), alkali hydrolyzed nitrogen (AN), available phosphorus (AP), and available potassium (AK). These findings suggest that loquat-tea intercropping promotes microbial diversity and enhances functional potential, improving soil health and nutrient availability in tea cultivation systems.
间作系统可显著影响土壤微生物群落，进而影响植物健康和土壤养分循环，相较于茶树单作具有更好的经济和生态效益。
本研究调查了枇杷 - 茶树间作对茶树根际土壤微生物群落结构和功能基因组成的影响。通过宏基因组测序，我们分析了枇杷 - 茶树间作（PP_CS）、茶树单作（CS）和枇杷单作（PP）的根际土壤。
各样本共注释出161个门、269个纲、485个目、1000个科、3838个属和27624个种。优势门包括 、 、 和 。 属（4.20%）和 属（3.78%）在间作系统中显著富集。群落差异分析表明，未分类的_c_酸杆菌在pp_cs组中，LDA得分最高（4.4分）。通过京都基因与基因组百科全书（KEGG）进行功能注释显示，所有处理中代谢途径占主导地位，有36111608条 reads 被分配到代谢。KEGG三级水平的比较分析表明，289 构成了所有三组中注释最丰富的功能类别。冗余分析（RDA）表明关键微生物属（ 、 ）与土壤性质之间存在强相关性，包括有机质（OM）、碱解氮（AN）、有效磷（AP）和速效钾（AK）。这些发现表明，枇杷 - 茶树间作促进了微生物多样性并增强了功能潜力，改善了茶树种植系统中的土壤健康和养分有效性。
