Zhang Xiaofeng, Fang Fazhi, Sha Linhua, Nizamani Mir Muhammad
Hainan Academy of Forestry (Hainan Academy of Mangrove), Haikou, China.
Key Laboratory of Tropical Forestry Resources Monitoring and Application of Hainan Province, Haikou, China.
Front Microbiol. 2025 Jul 3;16:1611262. doi: 10.3389/fmicb.2025.1611262. eCollection 2025.
, a resilient plant species found in the coastal ecosystems of Hainan Province, thrives in nutrient-poor, isolated habitats. This study examines the microbial community composition and ecological dynamics of across several islands in the South China Sea, aiming to understand how environmental factors like soil pH, nitrogen (N), and phosphorus (P) availability influence the plant's microbial communities and ecological success. The study was conducted across seven plots located on different islands, including Zhaoshu Island, Yongxing Island, and Wuzhizhou Island, among others. Soil samples were collected from three layers: 0-10 cm, 10-20 cm, and 20-30 cm. A total of 63 soil samples were processed for physicochemical analysis, measuring pH, organic matter, total nitrogen (TN), total phosphorus (TP), available phosphorus (AP), available potassium (AK), ammonium nitrogen ( ), and nitrate nitrogen ( ). Microbial DNA was extracted, amplified, and sequenced using Illumina-based platforms, producing 5,272,525 high-quality bacterial sequences and 5,273,089 fungal sequences. Bacterial communities were primarily composed of Proteobacteria (range: 29-50%), Planctomycetota, and Acidobacteriota, with significant correlations between certain bacterial genera and nutrient availability. Notably, ( = 0.810) and ( = 0.496) showed strong positive correlations with nitrogen availability. Fungal communities were dominated by Ascomycota, followed by Basidiomycota. ( = 0.585) and ( = 0.616) were positively correlated with nutrient availability. Diversity indices indicated that bacterial diversity was highest in pristine environments such as Zhaoshu Island (350 species), while fungal diversity was lower, with a maximum of 130 fungal species in less disturbed plots. The findings suggest that nutrient availability, particularly nitrogen and phosphorus, significantly shapes the microbial communities associated with . Bacterial diversity was positively correlated with nutrient-rich environments, while fungal diversity, especially Ascomycota, was more sensitive to disturbances, particularly in areas like Wuzhizhou Island and Yongxing Island, where human activity is higher. These results highlight the importance of maintaining nutrient availability and reducing human interference to preserve microbial diversity and the ecological resilience of in coastal ecosystems.
是一种生长在海南省沿海生态系统中的适应性强的植物物种，在营养贫瘠、孤立的栖息地中茁壮成长。本研究调查了中国南海多个岛屿上的微生物群落组成和生态动态，旨在了解土壤pH值、氮（N）和磷（P）有效性等环境因素如何影响该植物的微生物群落和生态成功。该研究在位于不同岛屿的七个地块上进行，包括赵述岛、永兴岛和蜈支洲岛等。从0 - 10厘米、10 - 20厘米和20 - 30厘米三个土层采集土壤样本。共对63个土壤样本进行了理化分析，测量了pH值、有机质、总氮（TN）、总磷（TP）、有效磷（AP）、有效钾（AK）、铵态氮（ ）和硝态氮（ ）。使用基于Illumina的平台提取、扩增和测序微生物DNA，产生了5,272,525条高质量细菌序列和5,273,089条真菌序列。细菌群落主要由变形菌门（范围：29 - 50%）、浮霉菌门和酸杆菌门组成，某些细菌属与养分有效性之间存在显著相关性。值得注意的是， （ = 0.810）和 （ = 0.496）与氮有效性呈强正相关。真菌群落以子囊菌门为主，其次是担子菌门。 （ = 0.585）和 （ = 0.616）与养分有效性呈正相关。多样性指数表明，在赵述岛等原始环境中细菌多样性最高（350种），而真菌多样性较低，在干扰较少的地块中最多有130种真菌。研究结果表明，养分有效性，特别是氮和磷，显著塑造了与 相关的微生物群落。细菌多样性与营养丰富的环境呈正相关，而真菌多样性，尤其是子囊菌门，对干扰更敏感，特别是在蜈支洲岛和永兴岛等人类活动较高的地区。这些结果凸显了维持养分有效性和减少人类干扰对于保护沿海生态系统中 的微生物多样性和生态恢复力 的重要性。
