Department of Genetics and Microbiology, Faculty of Science, Charles University, Vinicna 5, 128 44 Prague 2, Czechia.
Epidemiology and Ecology of Microorganisms, Crop Research Institute, Drnovska 507, Prague 6, Czechia.
Int J Syst Evol Microbiol. 2020 Sep;70(9):5106-5114. doi: 10.1099/ijsem.0.004388.
A novel actinobacterial strain, designated 15TR583, was isolated from a waterlogged acidic soil collected near the town of Trebon, Czech Republic, and was subjected to a polyphasic taxonomic characterization. Phylogenetic analysis based on 16S rRNA gene and whole-genome sequences revealed that the organism forms an individual line of descent related to the order , class . The strain shared highest 16S rRNA gene sequence similarity, yet of only 92.8%, with IFO 14752. The strain grew in white colonies of aerobic, Gram-stain-positive, unbranching substrate mycelium bearing single spores at hyphae tips. The major fatty acids (>10%) were iso-C, C, isoCω9 and 10-methyl-C. The fatty acid pattern differed from all patterns currently described for actinobacterial genera. The organism contained as major menaquinones MK9(H) and MK9(H), which differentiated it from other actinobacterial families. Polar lipids were composed of six unidentified glycolipids, an unidentified phosphoglycolipid, two unidentified phospholipids and two unidentified aminolipids. Whole-cell sugars contained galactose, xylose and arabinose as major components. The peptidoglycan type was A1γ -diaminopimelic acid. The genomic DNA G+C content was 69.7 mol%. The distinct phylogenetic position and unusual combination of chemotaxonomic characteristics justify the proposal of gen. nov., with the type species sp. nov. (type strain 15TR583=CCM 8942=DSM 109105), within fam. nov.
一株新的放线菌菌株，命名为 15TR583，从捷克特雷邦镇附近采集的水涝酸性土壤中分离得到，并进行了多相分类鉴定。基于 16S rRNA 基因和全基因组序列的系统发育分析表明，该菌形成了一个与目、纲相关的单系进化枝。该菌株与 IFO 14752 的 16S rRNA 基因序列相似性最高，为 92.8%。该菌株在需氧、革兰氏阳性、不分枝的基质菌丝上生长，形成白色菌落，菌丝顶端有单个孢子。主要脂肪酸（>10%）为 iso-C、C、isoCω9 和 10-甲基-C。脂肪酸模式与目前描述的放线菌属的所有模式都不同。该菌含有主要的menaquinones MK9(H) 和 MK9(H)，这将其与其他放线菌科区分开来。极性脂由六种未鉴定的糖脂、一种未鉴定的磷酸糖脂、两种未鉴定的磷脂和两种未鉴定的胺脂组成。全细胞糖含有半乳糖、木糖和阿拉伯糖作为主要成分。肽聚糖类型为 A1γ -二氨基庚二酸。基因组 DNA G+C 含量为 69.7 mol%。独特的系统发育位置和化学分类特征的不寻常组合证明了提议的属的合理性，新种，即 sp. nov.（模式菌株 15TR583=CCM 8942=DSM 109105），属于新科。
