Park Y B, Jahani M, Lacko A G
Comp Biochem Physiol B. 1985;82(3):529-33. doi: 10.1016/0305-0491(85)90018-5.
High density lipoprotein (HDL) was isolated from hog plasma by a simple immunoaffinity column chromatography procedure using immobilized anti-apolipoprotein AI. The composition of HDL isolated by immunoaffinity chromatography was nearly identical to that of a control sample that was isolated by an alternate method utilizing ultracentrifugation and gel chromatography. The HDL isolated by immunoaffinity chromatography had a larger number of polypeptide components that the control as indicated by acrylamide gel electrophoresis in the presence of urea. When the HDL isolated by immunoaffinity chromatography was applied to a heparin-agarose column the amount of protein retained was approximately twice that of the control. These findings indicate that the ultracentrifugation procedure probably induced the loss of apolipoprotein E containing components from the HDL complex.
通过使用固定化抗载脂蛋白AI的简单免疫亲和柱色谱法从猪血浆中分离出高密度脂蛋白（HDL）。通过免疫亲和色谱法分离的HDL的组成与通过利用超速离心和凝胶色谱法的另一种方法分离的对照样品的组成几乎相同。如在尿素存在下的丙烯酰胺凝胶电泳所示，通过免疫亲和色谱法分离的HDL比对照具有更多的多肽组分。当将通过免疫亲和色谱法分离的HDL应用于肝素 - 琼脂糖柱时，保留的蛋白质量约为对照的两倍。这些发现表明超速离心程序可能导致HDL复合物中含载脂蛋白E的组分丢失。
