Jahani M, Huttash R G, Lacko A G
Prep Biochem. 1980;10(4):431-44. doi: 10.1080/00327488008061741.
High density lipoproteins (HDL) were isolated by a procedure employing polyanion precipitation and column chromatography. The product polyanion precipitation and column chromatography. The product was free of low denisty lipoproteins (LDL) but serum albumin (HSA) was still present. The remaining HSA was removed by an immunoadsorbent column. The HDL isolated by our method was compared to another HDL preparation isolated from the same plasma sample by the combination of ultracentrifugation and gel chromatgraphy. It was found to have approximately the same lipid and protein composition as the HDL isolated by conventional techniques. Minor differences included a higher phospholipid and apoprotein E content and lower triglyceride and ApoC II content of the HDL isolated by column chromatography. The method described here is considerably less tedious than earlier techniques, can be scaled up without substantial increase in labor and results in an approximately 30% higher yield than the method described by Rudel et al.
通过采用聚阴离子沉淀和柱色谱法分离高密度脂蛋白（HDL）。产物经聚阴离子沉淀和柱色谱法处理。该产物不含低密度脂蛋白（LDL），但仍存在血清白蛋白（HSA）。通过免疫吸附柱去除剩余的HSA。将我们方法分离的HDL与通过超速离心和凝胶色谱法从同一血浆样品中分离的另一种HDL制剂进行比较。发现其脂质和蛋白质组成与通过传统技术分离的HDL大致相同。微小差异包括通过柱色谱法分离的HDL具有较高的磷脂和载脂蛋白E含量以及较低的甘油三酯和载脂蛋白C II含量。此处描述的方法比早期技术繁琐程度大大降低，可以在不大量增加劳动力的情况下扩大规模，并且比Rudel等人描述的方法产量高出约30%。
