Li H R
Zhonghua Zhong Liu Za Zhi. 1985 Jul;7(4):266-7.
On the basis of transmission electron microscopy of the gastric cancer, 10 cases with various types of the same tumor were further observed by Hitachii S-450 scanning electron microscope (SEM). The mucosa of the gastric cancer, para-cancer area and the perigastric lymph nodes were taken and fixed with penta-aldehyde and osmic acid, then processed into scanning specimens. By SEM, the para-cancer mucosa was quite similar to the "normal" gastric mucosa. Its free surface looked like strawberry. The gastric papule and fovea could be seen and the border of the cells was distinct. There was an uneven spacial structure similar to mountains and valleys at the border between the mucosa of the cancer and the non-cancer area. The mucosa of gastric adenocarcinoma showed wornout cotton-like erosion and destruction losing the normal tissue structures. The mucous epithelium was arranged disorderly adhered with marked dyskaryotic cancer cells. In the 2 cases of poorly differentiated adenocarcinoma and 1 undifferentiated carcinoma, metastatic cancer cells were found in the marginal sinuses of the perigastric lymph nodes. The scanogram of SEM showed a cluster of metastatic cancer cells neared and were adhering to the target organ. Some of the cells were observed stretching out their filament pseudopodia and invading the target organ. It implies a subsequent distant metastasis. Further study on the stereostructural changes of the surface membrane of cancer cells would help to explain the pathogenesis and development of gastric cancer.
在对胃癌进行透射电子显微镜观察的基础上，选取10例同一肿瘤不同类型的病例，用日立S - 450扫描电子显微镜（SEM）进行进一步观察。取胃癌组织、癌旁组织及胃周淋巴结的黏膜，用戊二醛和锇酸固定，然后制成扫描标本。通过扫描电子显微镜观察发现，癌旁黏膜与“正常”胃黏膜颇为相似。其游离面形似草莓。可见胃小丘和胃小凹，细胞边界清晰。癌组织与非癌组织交界处的空间结构高低不平，类似山峦和山谷。胃腺癌黏膜呈现出如磨损棉花般的糜烂和破坏，失去了正常组织结构。黏液上皮排列紊乱，黏附有明显核异型的癌细胞。在2例低分化腺癌和1例未分化癌中，在胃周淋巴结的边缘窦中发现了转移癌细胞。扫描电子显微镜扫描图显示，一群转移癌细胞靠近并附着于靶器官。观察到部分细胞伸出丝状伪足并侵入靶器官。这意味着随后会发生远处转移。对癌细胞表面膜立体结构变化的进一步研究将有助于解释胃癌的发病机制和发展过程。
