Kinoshita H, Tanaka E, Yoshida T, Kuroiwa Y
Eur J Drug Metab Pharmacokinet. 1985 Jul-Sep;10(3):247-51. doi: 10.1007/BF03189749.
Treatment of rats with phenobarbital (PB) caused an increase in microsomal biphenyl 4-hydroxylase activity and urinary glucaric acid excretion. Hepatic microsomal biphenyl 4-hydroxylase activity was correlative with urinary glucaric excretion in PB-treated rats. Hepatic microsomal biphenyl 2-hydroxylase activity was not correlated with urinary glucaric excretion in PB, 3-methylcholanthrene (3-MC)- and beta-naphthoflavone (beta-NF)-treated rats. Pretreatment of rats with carbon tetrachloride caused decreases in urinary glucaric acid excretion and biphenyl 2- and 4-hydroxylase activities. On the other hand, pretreatment with cobalt chloride caused decreases of these enzyme activities, but not of urinary glucaric acid excretion. These findings suggest that the urinary glucaric acid level would not always provide an index for assessment of hepatic drug metabolizing enzyme activity. These findings suggest that the urinary glucaric acid level would not always provide an index for assessment of hepatic drug metabolizing enzyme activity.
用苯巴比妥（PB）处理大鼠会导致微粒体联苯4-羟化酶活性增加以及尿中葡糖醛酸排泄量增加。在经PB处理的大鼠中，肝脏微粒体联苯4-羟化酶活性与尿中葡糖醛酸排泄相关。在经PB、3-甲基胆蒽（3-MC）和β-萘黄酮（β-NF）处理的大鼠中，肝脏微粒体联苯2-羟化酶活性与尿中葡糖醛酸排泄不相关。用四氯化碳预处理大鼠会导致尿中葡糖醛酸排泄量以及联苯2-和4-羟化酶活性降低。另一方面，用氯化钴预处理会导致这些酶活性降低，但不会导致尿中葡糖醛酸排泄量降低。这些发现表明，尿中葡糖醛酸水平并不总是能为评估肝脏药物代谢酶活性提供指标。这些发现表明，尿中葡糖醛酸水平并不总是能为评估肝脏药物代谢酶活性提供指标。
