Chen Wen, Liu Xinliang, Wu Zhenheng, Tan Haifen, Yu Fuqian, Wang Dongmei, Lin Xiaodan, Chen Zhigang
Department of Hepatobiliary Surgery, Fuzhou First General Hospital Affiliated of Fujian Medical University, Fuzhou, 350009, China.
Department of Radiation Oncology, Affiliated Changzhou No.2 People's Hospital of Nanjing Medical University, The Third Affiliated Hospital of Nanjing Medical University, Changzhou Medical Center, Nanjing Medical University, Changzhou, 213000, Jiangsu, China.
Biomed Eng Online. 2025 Aug 26;24(1):103. doi: 10.1186/s12938-025-01431-3.
Colorectal cancer (CRC) is a highly aggressive and extensive malignancy. Although long noncoding RNAs (lncRNAs) are often used as diagnostic biomarkers, their diagnostic effectiveness in CRC remains uncertain.
From January 1, 2015, to April 1, 2024, we conducted a comprehensive search of Embase, China National Knowledge Infrastructure (CNKI), Wanfang, PubMed, Cochrane Library, and Web of Science (WoS). The pooled sensitivity, specificity, positive likelihood ratio (PLR), negative likelihood ratio (NLR), diagnostic odds ratio (DOR), area under the receiver operating characteristic curve (AUC) and Fagan plot analysis were used to assess the overall test performance of lncRNAs. Moreover, we evaluated the publication bias using the Deeks' funnel plot asymmetry test.
Twenty-eight publications were identified and incorporated into this meta-analysis. The aggregated diagnostic data were as follows: The pooled sensitivity was 0.79 (95% CI, 0.75-0.83). The pooled specificity was 0.81 (95% CI, 0.78-0.84). The PLR was 3.68 (95% CI, 3.18-4.26). The NLR was 0.28 (95% CI, 0.24-0.33). The DOR was 15.01 (95% CI, 11.85-19.00). The AUC was 0.87 (95% CI, 0.84-0.90). Deeks' funnel plot asymmetry test indicated no significant evidence of publication bias (p > 0.05). The Fagan plot analysis showed that the post-test probability was 81% for positive results and 20% for negative results. Univariate meta-regression identified multiple sources of heterogeneity in the data, including year, sample size and specimen.
In summary, our findings demonstrate that lncRNAs have a promising diagnostic accuracy for CRC, underscoring their potential as effective non-invasive biomarkers.
结直肠癌（CRC）是一种侵袭性强且范围广泛的恶性肿瘤。尽管长链非编码RNA（lncRNAs）常被用作诊断生物标志物，但其在结直肠癌中的诊断有效性仍不确定。
从2015年1月1日至2024年4月1日，我们全面检索了Embase、中国知网（CNKI）、万方、PubMed、Cochrane图书馆和科学引文索引（WoS）。采用合并敏感度、特异度、阳性似然比（PLR）、阴性似然比（NLR）、诊断比值比（DOR）、受试者工作特征曲线下面积（AUC）和Fagan图分析来评估lncRNAs的整体检测性能。此外，我们使用Deeks漏斗图不对称性检验评估发表偏倚。
共纳入28篇文献进行此项荟萃分析。汇总的诊断数据如下：合并敏感度为0.79（95%CI，0.75 - 0.83）。合并特异度为0.81（95%CI，0.78 - 0.84）。PLR为3.68（95%CI，3.18 - 4.26）。NLR为0.28（95%CI，0.24 - 0.33）。DOR为15.01（95%CI，11.85 - 19.00）。AUC为0.87（95%CI，0.84 - 0.90）。Deeks漏斗图不对称性检验表明无显著的发表偏倚证据（p>0.05）。Fagan图分析显示，阳性结果的验后概率为81%，阴性结果的验后概率为20%。单因素meta回归确定了数据中的多个异质性来源，包括年份、样本量和标本类型。
总之，我们的研究结果表明lncRNAs对结直肠癌具有良好的诊断准确性，突出了它们作为有效的非侵入性生物标志物的潜力。
