Nikkhouy-Tanha Asmaalsadat, Nazari Nazanin, Moradi Maryam, Talebi Mahsa, Masoomi Sarvestani Fatima, Rahmanzad Fatemeh, Mahmoudi Maryam, Jafarinia Zahra, Rastegari Banafsheh
Department of Plant Protection, College of Agricultural Biotechnology Sciences, Shahed University, Tehran, Iran.
Department of Immunology, Shiraz University of Medical Sciences, Shiraz, Iran.
Adv Biomed Res. 2025 Jul 21;14:65. doi: 10.4103/abr.abr_419_24. eCollection 2025.
Prostate cancer is ranked as the most widespread malignancy, among men with the second level of mortality in the United States after lung cancer. Despite the low frequency of prostate cancer incidence in Iran, it has been informed as the fourth most predominant cancer among men with 6% of all deaths from cancer. Recent studies suggest that several hot spot points are indirectly associated with the incidence of prostate neoplasm. Our study aimed to test whether two different ITGBL1 and LPPR4/PRG1 gene expression levels are linked with prostate cancer incidence.
40 prostate tissue biopsies representing confirmed cancer and 41 normal tissues were selected. RNA extraction, cDNA synthesis, and TaqMan Real-time PCR were done according to the standard guidelines.
Among all 81 biopsy samples that were analyzed, expression levels of ITGBL1 have been significantly elevated in patients with prostate cancer. Also, the results of the present study showed that there was no significant correlation between LPPR4/PRG1 expression levels (2.79 ± 3.83) with prostate cancer incidence, while there has been no association between LPPR4/PRG1 expression levels (1.85 ± 1.55) in a patient with prostate cancer history out of control.
According to the role of ITGBL1 in several tumor phenotypes, we cannot ignore the importance of ITGBL1 genes as one of the critical regulatory proteins in prostate cancer development or progression. Therefore, more studies are required to explore the associations of prostate cancer with these regulatory proteins in more Iranian populations.
前列腺癌是最常见的恶性肿瘤之一，在美国男性中死亡率仅次于肺癌，位居第二。尽管伊朗前列腺癌的发病率较低，但据报告它是男性中第四大主要癌症，占所有癌症死亡人数的6%。最近的研究表明，几个热点与前列腺肿瘤的发病率间接相关。我们的研究旨在测试两种不同的整合素β样蛋白1（ITGBL1）和脂多糖诱导蛋白4/前列腺素E受体γ1（LPPR4/PRG1）基因表达水平是否与前列腺癌的发病率相关。
选取40份代表确诊癌症的前列腺组织活检样本和41份正常组织。按照标准指南进行RNA提取、cDNA合成和TaqMan实时荧光定量PCR。
在所有分析的81份活检样本中，前列腺癌患者中ITGBL1的表达水平显著升高。此外，本研究结果表明，LPPR4/PRG1表达水平（2.79±3.83）与前列腺癌发病率之间无显著相关性，而在无对照的前列腺癌病史患者中，LPPR4/PRG1表达水平（1.85±1.55）之间也无关联。
根据ITGBL1在几种肿瘤表型中的作用，我们不能忽视ITGBL1基因作为前列腺癌发生或发展中关键调节蛋白之一的重要性。因此，需要更多的研究来探索在更多伊朗人群中前列腺癌与这些调节蛋白之间的关联。
