Yasir Mohammed Kareem, Minasian Vazgen, Hovsepian Silva, Hashemipour Mahin
Department of Exercise Physiology, Faculty of Sport Sciences, University of Isfahan, Isfahan, Iran.
Metabolic Liver Disease Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
Adv Biomed Res. 2025 Jul 31;14:71. doi: 10.4103/abr.abr_105_24. eCollection 2025.
At present, increasing evidence has reported circulating microRNAs (miRNAs) as potential clinical biomarkers for specific diseases and administration of pharmaceutical agents. The main aim of this study was to compare the effect of two selected home-based resistance exercise (HBRE) and game-based exercise training (GBET) groups on microRNA21-5p expression and biomarkers related to non-alcoholic fatty liver in children with type 1 diabetes (T1D).
Twenty children with T1D aged 10-15 years were randomly assigned to one of two groups (HBRE: n = 10; GBET: n = 10). The exercise training lasted for 8 weeks with three sessions per week. The liver enzyme profile, hemoglobin A1c (HbA1c), glucose, microRNA-21-5p, insulin, and Homeostasis Model Assessment for Insulin Resistance (HOMA-IR) levels were measured before and after the interventions in both groups. In addition, the cardiopulmonary fitness and body fat percentage (BF%) of the participants were also evaluated.
After exercise interventions, miR-21-5p increased significantly for both intervention groups (HBRE: 8.9% vs. GBET: 6.7%; = 0.001). The levels of alanine transaminase (ALT) (HBRE: 60.71% vs. GBET: 49.15%) and aspartate aminotransferase (AST) enzymes (HBRE: 54.02% vs. GBET: 34.02%), the glucose (HBRE: 15.03% vs. GBET: 16.26%), and the HbA1c (HBRE: -10.44% vs. GBET: -6.52%) were decreased significantly ( ≤ 0.05). The cardiorespiratory endurance of subjects increased (HBRE: 2.85% vs. GBET: 3.75%), but there were no differences between groups ( ≥ 0.05).
Both HBRE and GBET protocols were effective to improve cardiorespiratory fitness, liver enzyme profile, glycemic control, and miR-21-5p changes that seem to be indicative of the pathological status of T1D children.
目前，越来越多的证据表明，循环微RNA（miRNA）可作为特定疾病和药物治疗的潜在临床生物标志物。本研究的主要目的是比较两种选定的居家抗阻运动（HBRE）和游戏式运动训练（GBET）对1型糖尿病（T1D）儿童的微小RNA21-5p表达及与非酒精性脂肪肝相关生物标志物的影响。
20名年龄在10至15岁的T1D儿童被随机分为两组（HBRE组：n = 10；GBET组：n = 10）。运动训练持续8周，每周进行三次。在两组干预前后测量肝酶谱、糖化血红蛋白（HbA1c）、血糖、微小RNA-21-5p、胰岛素以及胰岛素抵抗稳态模型评估（HOMA-IR）水平。此外，还评估了参与者的心肺适能和体脂百分比（BF%）。
运动干预后，两个干预组的miR-21-5p均显著增加（HBRE组：8.9% 对比GBET组：6.7%；P = 0.001）。丙氨酸转氨酶（ALT）水平（HBRE组：60.71% 对比GBET组：49.15%）和天冬氨酸转氨酶（AST）水平（HBRE组：54.02% 对比GBET组：34.02%）、血糖（HBRE组：15.03% 对比GBET组：16.26%）以及HbA1c（HBRE组：-10.44% 对比GBET组：-6.52%）均显著降低（P≤0.05）。受试者的心肺耐力有所提高（HBRE组：2.85% 对比GBET组：3.75%），但两组之间无差异（P≥0.05）。
HBRE和GBET方案均能有效改善心肺适能、肝酶谱、血糖控制以及miR-21-5p的变化，这些变化似乎表明了T1D儿童的病理状态。
