Antwi-Boasiako Michael Nana, Ayensu Frederick, Awuah Aaron, Mutala Abdul-Hakim, Boampong Kwadwo, Badu Kingsley
Department of Theoretical and Applied Biology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
HopeXchange Medical Centre, Kumasi, Ghana.
Microbiologyopen. 2025 Oct;14(5):e70028. doi: 10.1002/mbo3.70028.
Urinary tract infections (UTIs) are one of the most prevalent infections in both men and women. The most common causative organisms are Escherichia coli, Klebsiella spp., and Staphylococcus spp. UTIs are diagnosed using various diagnostic methods with urine culture as the gold standard. This study aimed to estimate the prevalence of UTIs, compare the tools employed in the diagnosis of UTIs, and assess the antibiotic susceptibility pattern of bacterial isolates among patients in a local health facility in Kumasi, Ghana. A cohort of 208 participants were enlisted for a cross-sectional study at the HopeXchange Medical Centre, Kumasi. Mid-stream urine samples were collected from participants and analyzed by culture, microscopy, and dipstick methods. Culture isolates were tested on Mueller-Hinton Agar applying the Kirby Bauer method against a range of antibiotics. The prevalence of Staphylococcus spp. (25%) and Klebsiella spp. (16.7%) were higher than that of Escherichia coli (8.3%). UTI was more common in individuals between 50 and 59 years of age (36.5%) and mostly associated among women (76.9%). Overall, culture confirmed UTI prevalence was 23.08% while dispstick and microscopy diagnosed 44.23% and 47.60% of UTIs, respectively. Microscopy exhibited a sensitivity of 68.8% and specificity of 58.8% while the dipstick had a sensitivity of 60.4% and specificity of 60.6% compared to urine culture. Meropenem showed 100% susceptibility, while high resistance was observed for chloramphenicol and ceftriaxone against Escherichia coli, Staphylococcus spp., Providencia spp., and Klebsiella spp. UTI was significantly associated with age, gender, and the standard diagnostic method (p < 0.05).
尿路感染（UTIs）是男性和女性中最常见的感染之一。最常见的病原体是大肠杆菌、克雷伯菌属和葡萄球菌属。尿路感染采用多种诊断方法进行诊断，其中尿培养是金标准。本研究旨在估计加纳库马西当地一家医疗机构中患者尿路感染的患病率，比较用于诊断尿路感染的工具，并评估细菌分离株的抗生素敏感性模式。招募了208名参与者在库马西的HopeXchange医疗中心进行横断面研究。从参与者中采集中段尿样本，并通过培养、显微镜检查和试纸法进行分析。培养分离株在穆勒-欣顿琼脂上采用 Kirby Bauer 方法针对一系列抗生素进行测试。葡萄球菌属（25%）和克雷伯菌属（16.7%）的患病率高于大肠杆菌（8.3%）。尿路感染在50至59岁的个体中更为常见（36.5%），且大多发生在女性中（76.9%）。总体而言，培养确诊的尿路感染患病率为23.08%，而试纸法和显微镜检查分别诊断出44.23%和47.60%的尿路感染。与尿培养相比，显微镜检查的敏感性为68.8%，特异性为58.8%，而试纸法的敏感性为60.4%，特异性为60.6%。美罗培南显示出100%的敏感性，而氯霉素和头孢曲松对大肠杆菌、葡萄球菌属、普罗威登斯菌属和克雷伯菌属表现出高耐药性。尿路感染与年龄、性别和标准诊断方法显著相关（p < 0.05）。
