Aiba Hisaki, Kimura Hiroaki, Terauchi Ryu, Suzuki Nobuyuki, Kato Kenji, Yagi Kiyoshi, Yamaguchi Makoto, Murakami Kiyoka, Suenaga Shogo, Shirai Toshiharu, Aso Ayano, Errani Costantino, Murakami Hideki
Department of Orthopedic Surgery, Graduate School of Medical Sciences, Nagoya City University, Nagoya 467-8601, Japan.
Department of Orthopaedics, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, 465 Kajii-cho, Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto 602-8566, Japan.
Curr Oncol. 2025 Jul 31;32(8):432. doi: 10.3390/curroncol32080432.
Cryotherapy could stimulate immune responses and induce abscopal effects. A novel technique was developed for treating spinal bone tumors involving the use of frozen tumor-containing autologous bone grafts for anterior spinal reconstruction following total en-bloc spondylectomy, with the aim of activating cryoimmunity. This study focused on analyzing changes in the T-cell receptor (TCR) repertoire after surgery to evaluate T-cell diversity. Blood samples were collected pre- and post-operatively, with subsequent RNA extraction and immunosequencing. Compared to pre-surgery samples, the diversity and abundance of the Complementarity-Determining Region 3, regions of the TCR α and β chains decreased, suggesting that more selective clones may have emerged and influenced immune responses. Through TCR repertoire analysis, this study demonstrated that transplantation of frozen tumor-containing autologous bone impacted the immune system. This study is expected to provide a foundation for developing treatments that may enhance immune activation.
冷冻疗法可刺激免疫反应并诱导远隔效应。一种新的技术被开发用于治疗脊柱骨肿瘤，该技术涉及在整块全脊椎切除术后使用含冷冻肿瘤的自体骨移植进行前路脊柱重建，目的是激活冷冻免疫。本研究着重分析术后T细胞受体（TCR）库的变化，以评估T细胞多样性。术前和术后采集血样，随后进行RNA提取和免疫测序。与术前样本相比，TCRα和β链的互补决定区3的多样性和丰度降低，这表明可能出现了更多选择性克隆并影响了免疫反应。通过TCR库分析，本研究表明含冷冻肿瘤的自体骨移植影响了免疫系统。本研究有望为开发可能增强免疫激活的治疗方法提供基础。
