Kausaite-Minkstimiene Asta, Krikstaponyte Aiste, Stasyuk Nataliya, Gayda Galina, Ramanaviciene Almira
NanoTechnas-Center of Nanotechnology and Materials Science, Faculty of Chemistry and Geosciences, Vilnius University, Naugarduko st. 24, LT-03225 Vilnius, Lithuania.
Department of Analytical Biotechnology, Institute of Cell Biology National Academy of Sciences of Ukraine (ICB NASU), Dragomanova Str. 14/16, 79005 Lviv, Ukraine.
Biosensors (Basel). 2025 Aug 19;15(8):545. doi: 10.3390/bios15080545.
Amperometric biosensors, due to their high sensitivity, fast response time, low cost, simple control, miniaturization capabilities, and other advantages, are receiving significant attention in the field of medical diagnostics, especially in monitoring blood glucose levels in diabetic patients. In this study, an amperometric glucose biosensor based on immobilized enzyme glucose oxidase (GOx) and bimetallic platinum cobalt (PtCo) nanoparticles was developed. The PtCo nanoparticles, deposited on a graphite rod electrode, exhibited peroxidase-like catalytic properties and were able to electrocatalyze the reduction of HO. After immobilization of the GOx, an amperometric signal generated by the biosensor was directly proportional to the glucose concentration in the range of 0.04-2.18 mM. The biosensor demonstrated a sensitivity of 19.38 μA mM cm, with a detection limit of 0.021 mM and a quantification limit of 0.064 mM. In addition to this analytical performance, the biosensor exhibited excellent repeatability (relative standard deviation (RSD) was 4.90%); operational and storage stability, retaining 98.93% and 95.33% of its initial response after 26 cycles of glucose detection and over a 14-day period, respectively; and anti-interference ability against electroactive species, as well as exceptional selectivity for glucose and satisfactory reproducibility (RSD 8.90%). Additionally, the biosensor was able to detect glucose levels in blood serum with a high accuracy (RSD 5.89%), indicating potential suitability for glucose determination in real samples.
安培生物传感器因其高灵敏度、快速响应时间、低成本、简单控制、小型化能力等优点，在医学诊断领域受到了广泛关注，特别是在监测糖尿病患者的血糖水平方面。在本研究中，开发了一种基于固定化酶葡萄糖氧化酶（GOx）和双金属铂钴（PtCo）纳米颗粒的安培型葡萄糖生物传感器。沉积在石墨棒电极上的PtCo纳米颗粒表现出类似过氧化物酶的催化特性，能够电催化HO的还原。固定GOx后，生物传感器产生的安培信号在0.04 - 2.18 mM范围内与葡萄糖浓度成正比。该生物传感器的灵敏度为19.38 μA mM cm，检测限为0.021 mM，定量限为0.064 mM。除了这种分析性能外，该生物传感器还表现出优异的重复性（相对标准偏差（RSD）为4.90%）；操作和储存稳定性，在26次葡萄糖检测循环后和14天内分别保留其初始响应的98.93%和95.33%；对电活性物质的抗干扰能力，以及对葡萄糖的优异选择性和令人满意的重现性（RSD 8.90%）。此外，该生物传感器能够高精度地检测血清中的葡萄糖水平（RSD 5.89%），表明其在实际样品中测定葡萄糖具有潜在的适用性。
