Pautova Alisa K, Meinarovich Peter A, Zakharchenko Vladislav E, Sobolev Pavel D, Burnakova Natalia A, Beloborodova Natalia V
Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Petrovka Street, 25-2, 107031 Moscow, Russia.
Exacte Labs Bioanalytical Laboratory, 20-2 Nauchny Proezd, 117246 Moscow, Russia.
Metabolites. 2025 Aug 3;15(8):527. doi: 10.3390/metabo15080527.
Secondary (nosocomial) bacterial meningitis remains a serious problem in patients with severe brain damage. The aim of this study was to assess the differences in the aromatic metabolites of tryptophan, phenylalanine, and tyrosine, in serum and cerebrospinal fluid (CSF) samples collected simultaneously from patients with long-term sequelae of severe brain damage with suspected secondary bacterial meningitis. Group I included 16 paired serum and CSF samples from patients ( = 11) without secondary bacterial meningitis; group II included 13 paired serum and CSF samples from patients ( = 4) with secondary bacterial meningitis. The median concentrations of serum 5-hydroxyindole-3-acetic, CSF 4-hydroxyphenyllactic (-HPhLA), CSF 4-hydroxyphenylacetic, CSF phenyllactic, and indole-3-lactic acids in serum and CSF were statistically higher in group II compared to group I (-value ≤ 0.03), while 4-hydroxyphenylpropionic and indole-3-acetic in serum were lower in group II compared to group I (-value = 0.04). In group I, -HPhLA serum concentrations were greater than or equal to its CSF concentrations in 14 paired samples; in group II, -HPhLA concentrations in serum were lower than in CSF in all paired samples. The obtained results demonstrate the differences in the profile of aromatic metabolites in serum and CSF and may confirm the hypothesis of the -HPhLA microbial origin in the CSF of patients with secondary bacterial meningitis.
继发性（医院获得性）细菌性脑膜炎在重度脑损伤患者中仍然是一个严重问题。本研究的目的是评估从患有重度脑损伤长期后遗症且疑似继发性细菌性脑膜炎的患者同时采集的血清和脑脊液（CSF）样本中色氨酸、苯丙氨酸和酪氨酸的芳香族代谢产物的差异。第一组包括16对来自无继发性细菌性脑膜炎患者（n = 11）的血清和脑脊液样本；第二组包括13对来自患有继发性细菌性脑膜炎患者（n = 4）的血清和脑脊液样本。与第一组相比，第二组血清中5-羟吲哚-3-乙酸、脑脊液中4-羟基苯乳酸（4-HPhLA）、脑脊液中4-羟基苯乙酸、脑脊液中苯乳酸以及血清和脑脊液中的吲哚-3-乳酸的中位数浓度在统计学上更高（p值≤0.03），而第二组血清中的4-羟基苯丙酸和吲哚-3-乙酸低于第一组（p值 = 0.04）。在第一组中，14对样本的血清4-HPhLA浓度大于或等于其脑脊液浓度；在第二组中，所有配对样本的血清4-HPhLA浓度均低于脑脊液浓度。获得的结果表明血清和脑脊液中芳香族代谢产物谱存在差异，并可能证实继发性细菌性脑膜炎患者脑脊液中4-HPhLA微生物来源的假设。
