Moggio M, Bonilla E
Histochemistry. 1985;83(6):519-23. doi: 10.1007/BF00492454.
Polymyxin B was used as a probe to label anionic phospholipids in skeletal muscle plasma membrane. This antibiotic produces muscle surface membrane lesions that can be identified in both thin sections and freeze-fracture replicas. The membrane perturbations assumed a patchy distribution with a preferential localization at the level of the I band and A-I bands junction. Intramembraneous particles were also observed within the lesions. We consider the possibility that microdomains of anionic phospholipids in muscle plasma membrane may function in the binding of Ca++.
多粘菌素B被用作探针来标记骨骼肌质膜中的阴离子磷脂。这种抗生素会造成肌肉表面膜损伤，在超薄切片和冷冻蚀刻复制品中均可识别。膜扰动呈现出斑块状分布，优先定位于I带和A-I带交界处。在损伤部位还观察到了膜内颗粒。我们考虑了骨骼肌质膜中阴离子磷脂微区可能在钙离子结合中发挥作用的可能性。
