Hoogenboom J J, Rammell C G
J Assoc Off Anal Chem. 1985 Nov-Dec;68(6):1131-3.
A chloroform extract of stomach contents at basic pH is concentrated and then extracted with 0.1 M phosphoric acid. The acid extract is chromatographed on a 10 cm reverse phase column, using 0.005 M phosphate buffer (pH 3.0)-acetonitrile-tetrahydrofuran (750 + 135 + 115) containing 0.01 M octanesulfonic acid at a flow rate of 1.0 mL/min for elution. Strychnine eluted in 7.3 min. Recoveries from spiked stomach contents averaged 92%. The method can be used without modification for other alkaloids.
将碱性pH值的胃内容物氯仿提取物浓缩，然后用0.1 M磷酸萃取。酸提取物在10 cm反相柱上进行色谱分析，使用含0.01 M辛烷磺酸的0.005 M磷酸盐缓冲液（pH 3.0）-乙腈-四氢呋喃（750 + 135 + 115），流速为1.0 mL/min进行洗脱。士的宁在7.3分钟时洗脱。加标胃内容物的平均回收率为92%。该方法无需修改即可用于其他生物碱。
