Crouch M D, Short C R
J Assoc Off Anal Chem. 1978 May;61(3):612-5.
A simple, rapid extraction and subsequent determination for strychnine, using high pressure liquid chromatography (HPLC) with a reverse phase solvent system, is described. Stomach contents or grain bait containing strychnine were made alkaline with sodium hydroxide and extracted with chloroform. Extract filtrates were injected directly into a liquid chromatograph without further preparation except for dilution, if necessary. Peaks were resolved within 3.5 min and peak heights were used for quantitation. HPLC of strychnine was carried out on a 30 cm X 4 mm id stainless steel column packed with micronBondapak C18. The solvent program was 0.005M phosphate buffer-methanol (60+40) at a flow of 1.5 ml/min. Recovery from spiked stomach contents was 93.9 +/- 3.5%. The detection capability for strynchnine, using the 254 nm ultraviolet detector, was 5 ng. The strychnine peak was collected and subjected to thin layer chromatography with strychnine standards for confirmation.
本文描述了一种简单、快速的马钱子碱提取及后续测定方法，该方法采用反相溶剂系统的高压液相色谱（HPLC）。含马钱子碱的胃内容物或谷物诱饵用氢氧化钠碱化后，用氯仿萃取。萃取滤液除必要时稀释外，无需进一步处理，可直接注入液相色谱仪。3.5分钟内可分离出峰，并使用峰高进行定量。马钱子碱的HPLC分析在一根30 cm×4 mm内径、填充有微孔键合硅胶C18的不锈钢柱上进行。溶剂程序为0.005M磷酸盐缓冲液 - 甲醇（60 + 40），流速为1.5 ml/min。加标胃内容物的回收率为93.9 +/- 3.5%。使用254 nm紫外检测器时，马钱子碱的检测限为5 ng。收集马钱子碱峰，并用马钱子碱标准品进行薄层色谱法确证。
