Belal F
J Assoc Off Anal Chem. 1985 Nov-Dec;68(6):1207-9.
A flow-injection method is described for the determination of phenazopyridine hydrochloride, based on electrochemical oxidation at the glassy carbon electrode. The suggested method is highly specific and can be used to determine phenazopyridine HCl in the presence of most drugs commonly found in pharmaceutical dosage forms or administered therapeutically. Applying a constant potential of +950 mV vs Ag/AgCl/3.5M KCl reference electrode, the calibration curve was linear in the 1-30 micrograms/mL range, with minimum detectability of 0.2 ng (signal-to-noise ratio 2). Good accuracy and precision were obtained when the method was applied to some dosage forms containing phenazopyridine HCl. Although automation was not used in this study, an automated system could be incorporated because the method uses the technique of continuous analysis in a flowing stream.
描述了一种基于玻碳电极上的电化学氧化测定盐酸非那吡啶的流动注射法。所建议的方法具有高度特异性，可用于在药物剂型中常见或治疗给药的大多数药物存在下测定盐酸非那吡啶。相对于Ag/AgCl/3.5M KCl参比电极施加+950 mV的恒定电位，校准曲线在1-30微克/毫升范围内呈线性，最低检测限为0.2纳克（信噪比2）。当该方法应用于一些含有盐酸非那吡啶的剂型时，获得了良好的准确度和精密度。尽管本研究未使用自动化，但由于该方法采用流动体系中的连续分析技术，因此可以纳入自动化系统。
