Adhoum Nafaâ, Monser Lotfi
Institut National des Sciences Appliquées et de Technologie, Département de Chimie, Centre Urbain Nord B.P.N 676, 1080 Tunis Cedex, Tunisia.
J Pharm Biomed Anal. 2005 Jul 15;38(4):619-23. doi: 10.1016/j.jpba.2005.02.021. Epub 2005 Apr 2.
The differential pulse voltammetric (DPV) determination of trimebutine (TMB) was achieved at a glassy carbon electrode in acetonitrile/0.1 M LiClO4. Trimebutine gave two irreversible, diffusion controlled peaks at 740 and 1318 mV versus Ag/AgCl reference electrode, respectively. The second oxidation peak was used to determine trimebutine concentrations in the range 1-50 microg ml(-1) with a detection limit (3sigmam) of 0.3 microg ml(-1). Precision of the method (RSD, n=6) within- and between-days obtained from six determinations at 5 microg ml(-1) was found to be 0.7 and 1.1%, respectively. The method was successfully applied to the quantitation of TMB in granule dosage form (Debridat) and recoveries between 98.4 and 101% were obtained. Excipients did not interfere with the assay and the results agreed well with those determined by previously established HPLC method.
在乙腈/0.1 M高氯酸锂体系中，于玻碳电极上采用差分脉冲伏安法（DPV）测定曲美布汀（TMB）。相对于Ag/AgCl参比电极，曲美布汀分别在740和1318 mV处给出两个不可逆的、受扩散控制的峰。利用第二个氧化峰测定曲美布汀的浓度范围为1 - 50 μg ml⁻¹，检测限（3σ）为0.3 μg ml⁻¹。从5 μg ml⁻¹的六个测定值得到的日内和日间方法精密度（RSD，n = 6）分别为0.7%和1.1%。该方法成功应用于颗粒剂型（Debridat）中TMB的定量分析，回收率在98.4%至101%之间。辅料不干扰测定，结果与先前建立的HPLC方法测定结果吻合良好。
