Parks O W
J Assoc Off Anal Chem. 1985 Nov-Dec;68(6):1232-4.
Thin layer chromatographic and liquid chromatographic procedures were used to show that sulfonamides containing a heterocyclic amine moiety and free N1 acidic hydrogen will photodegrade under fluorescent light in model systems containing riboflavin. The photodegradation product was characteristic of the drug. In-depth studies on sulfamethazine showed that the drug also photodegraded in the presence of lumichrome and flavin mononucleotide; the rate of photodegradation depended on the photosensitizer and its concentration. Crude polar liver extracts sensitized the photodegradation of sulfamethazine, but to a degree less than expected on the basis of reported riboflavin content of livers. It is recommended that procedures for quantitating sulfa drugs and their metabolites be performed in subdued lighting and/or that amber or low actinic vessels be used to prevent losses due to photochemical reactions.
采用薄层色谱法和液相色谱法表明，含有杂环胺部分和游离N1酸性氢的磺胺类药物在含有核黄素的模型系统中，在荧光灯下会发生光降解。光降解产物具有该药物的特征。对磺胺二甲嘧啶的深入研究表明，该药物在发光色素和黄素单核苷酸存在的情况下也会发生光降解；光降解速率取决于光敏剂及其浓度。粗制的极性肝脏提取物可使磺胺二甲嘧啶的光降解敏感化，但程度低于根据报道的肝脏核黄素含量所预期的程度。建议在较暗的光线下进行磺胺类药物及其代谢物的定量分析，和/或使用琥珀色或低光化性容器，以防止因光化学反应造成损失。
