Thomas M H, Soroka K E, Thomas S H
J Assoc Off Anal Chem. 1983 Jul;66(4):881-3.
In-situ optical scanning of fluorescamine derivatives on thin layer silica gel plates provides a rapid method for the determination of multiple sulfonamides at levels below 0.1 ppm. Sample preparation is minimal. Homogenized liver or muscle is extracted with ethyl acetate and then back-extracted into 0.2M glycine buffer. After pH adjustment, the extract is washed with hexane and extracted with methylene chloride. The organic phase is evaporated to dryness and reconstituted in methanol. Pre-adsorbent layer silica gel plates are used for chromatography. The method has been applied to residues of sulfamethazine, sulfadimethoxine, sulfathiazole, sulfaquinoxaline, and sulfabromomethazine in cattle, swine, turkey, and duck tissues.
在薄层硅胶板上对荧光胺衍生物进行原位光学扫描，为测定低于0.1 ppm水平的多种磺胺类药物提供了一种快速方法。样品制备过程极为简单。将匀浆后的肝脏或肌肉用乙酸乙酯萃取，然后反萃取至0.2M甘氨酸缓冲液中。调节pH值后，萃取液先用己烷洗涤，再用二氯甲烷萃取。有机相蒸发至干，然后用甲醇复溶。预吸附层硅胶板用于色谱分析。该方法已应用于牛、猪、火鸡和鸭组织中磺胺二甲嘧啶、磺胺二甲氧嘧啶、磺胺噻唑、磺胺喹恶啉和磺胺溴甲嘧啶的残留检测。
