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离子强度和巯基试剂对肌酸磷酸激酶与心脏线粒体内膜结合的影响。

Effects of ionic strength and sulfhydryl reagents on the binding of creatine phosphokinase to heart mitochondrial inner membranes.

作者信息

Wenger W C, Murphy M P, Brierley G P, Altschuld R A

出版信息

J Bioenerg Biomembr. 1985 Oct;17(5):295-303. doi: 10.1007/BF00751106.

DOI:10.1007/BF00751106
PMID:4086487
Abstract

The concept that creatine phosphokinase is bound to the outer surface of the heart mitochondrial inner membrane originated from observations that the enzyme is retained by water-swollen heart mitochondria and by digitonin-treated heart mitochondria suspended in isotonic sucrose. The present study establishes that digitonin-treated mitochondria release creatine phosphokinase in isotonic KCl, and other investigators have reported an identical response for the water-swollen organelles. These observations suggest that mitochondrial creatine phosphokinase is not bound to the outer surface of the inner membrane at a site adjacent to the adenine nucleotide translocase under physiologic conditions.

摘要

肌酸磷酸激酶与心脏线粒体内膜外表面结合的概念源于以下观察结果

该酶可被水膨胀的心脏线粒体以及悬浮在等渗蔗糖中的洋地黄皂苷处理过的心脏线粒体保留。本研究证实，洋地黄皂苷处理过的线粒体在等渗氯化钾中会释放肌酸磷酸激酶，其他研究人员也报道了水膨胀细胞器有相同的反应。这些观察结果表明，在生理条件下，线粒体肌酸磷酸激酶并非在与腺嘌呤核苷酸转位酶相邻的内膜外表面结合。

相似文献

1
Effects of ionic strength and sulfhydryl reagents on the binding of creatine phosphokinase to heart mitochondrial inner membranes.离子强度和巯基试剂对肌酸磷酸激酶与心脏线粒体内膜结合的影响。
J Bioenerg Biomembr. 1985 Oct;17(5):295-303. doi: 10.1007/BF00751106.
2
Compartmentation of mitochondrial creatine phosphokinase. II. The importance of the outer mitochondrial membrane for mitochondrial compartmentation.线粒体肌酸磷酸激酶的区室化。II. 线粒体外膜对于线粒体区室化的重要性。
J Biol Chem. 1982 Dec 10;257(23):14405-11.
3
Study on heart mitochondrial creatine kinase using a cross-linking bifunctional reagent. II. The binding sites for the creatine kinase octamer on mitochondrial membranes have different properties.使用交联双功能试剂对心脏线粒体肌酸激酶的研究。II. 线粒体膜上肌酸激酶八聚体的结合位点具有不同特性。
Biochem Int. 1989 Jun;18(6):1149-59.
4
[Interaction between mitochondrial creatine kinase and mitochondrial membranes].[线粒体肌酸激酶与线粒体膜之间的相互作用]
Biokhimiia. 1980 Jul;45(7):1155-66.
5
Heart mitochondria in physiological salt solution: not ionic strength but salt composition is important for association of creatine kinase with the inner membrane surface.生理盐溶液中的心脏线粒体：对于肌酸激酶与内膜表面的结合而言，重要的不是离子强度，而是盐的组成。
Biochem Biophys Res Commun. 1986 Sep 30;139(3):1262-71. doi: 10.1016/s0006-291x(86)80314-x.
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Heart mitochondrial creatine kinase revisited: the outer mitochondrial membrane is not important for coupling of phosphocreatine production to oxidative phosphorylation.再探心脏线粒体肌酸激酶：线粒体外膜对于磷酸肌酸生成与氧化磷酸化的偶联并不重要。
Arch Biochem Biophys. 1989 Jan;268(1):176-90. doi: 10.1016/0003-9861(89)90578-x.
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Creatine kinase of rat heart mitochondria. The demonstration of functional coupling to oxidative phosphorylation in an inner membrane-matrix preparation.大鼠心脏线粒体肌酸激酶。在内膜-基质制剂中与氧化磷酸化功能偶联的证明。
J Biol Chem. 1985 Jun 25;260(12):7757-64.
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Influence of the mitochondrial outer membrane and the binding of creatine kinase to the mitochondrial inner membrane on the compartmentation of adenine nucleotides in the intermembrane space of rat heart mitochondria.线粒体外膜以及肌酸激酶与线粒体内膜的结合对大鼠心脏线粒体膜间隙中腺嘌呤核苷酸分隔的影响。
Biochim Biophys Acta. 1993 Jan 8;1140(3):327-34. doi: 10.1016/0005-2728(93)90073-o.
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Adriamycin-mediated inhibition of creatine phosphokinase binding to heart mitochondrial membrane.
Biochem Pharmacol. 1982 Jan 1;31(1):109-11. doi: 10.1016/0006-2952(82)90245-3.
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Sub-mitochondrial and sub-microsomal distribution of creatine kinase in guinea pig myocardium.
Cardiovasc Res. 1979 Oct;13(10):562-7. doi: 10.1093/cvr/13.10.562.

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The influence of the cytosolic oncotic pressure on the permeability of the mitochondrial outer membrane for ADP: implications for the kinetic properties of mitochondrial creatine kinase and for ADP channelling into the intermembrane space.胞质胶体渗透压对线粒体外膜对ADP通透性的影响：对线粒体肌酸激酶动力学特性及ADP进入膜间隙的影响
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本文引用的文献

1
Binding of creatine kinase to heart and liver mitochondria in vitro.肌酸激酶在体外与心脏和肝脏线粒体的结合
Arch Biochem Biophys. 1980 May;201(2):674-7. doi: 10.1016/0003-9861(80)90558-5.
2
Heart mitochondrial creatine kinase solubilization. Effect of mitochondrial swelling and SH group reagents.心脏线粒体肌酸激酶的溶解。线粒体肿胀和巯基试剂的作用。
Arch Biochem Biophys. 1981 Nov;212(1):195-203. doi: 10.1016/0003-9861(81)90359-3.
3
[Interactions between heart mitochondrial creatine kinase and oxidative phosphorylation].[心脏线粒体肌酸激酶与氧化磷酸化之间的相互作用]
Biokhimiia. 1980 Aug;45(8):1347-51.
4
[Interaction between mitochondrial creatine kinase and mitochondrial membranes].[线粒体肌酸激酶与线粒体膜之间的相互作用]
Biokhimiia. 1980 Jul;45(7):1155-66.
5
Studies of the interaction of mitochondrial creatine kinase with the mitochondrial membrane.
Adv Myocardiol. 1982;3:597-611. doi: 10.1007/978-1-4899-5561-6_57.
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Release of enzymes from adult rat heart myocytes.
Circ Res. 1982 Nov;51(5):560-8. doi: 10.1161/01.res.51.5.560.
7
Control of heart mitochondrial oxygen consumption by creatine kinase: the importance of enzyme localization.肌酸激酶对心脏线粒体氧消耗的调控：酶定位的重要性。
Biochem Biophys Res Commun. 1982 Apr 29;105(4):1473-81. doi: 10.1016/0006-291x(82)90954-8.
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Effects of SH group reagents on creatine kinase interaction with the mitochondrial membrane.
Arch Biochem Biophys. 1983 Feb 1;220(2):541-8. doi: 10.1016/0003-9861(83)90445-9.
9
A simple analysis of the "phosphocreatine shuttle".“磷酸肌酸穿梭”的简单分析
Am J Physiol. 1984 May;246(5 Pt 1):C365-77. doi: 10.1152/ajpcell.1984.246.5.C365.
10
Effect of creatine kinase activity on mitochondrial ADP/ATP transport. Evidence for a functional interaction.肌酸激酶活性对线粒体ADP/ATP转运的影响。功能相互作用的证据。
J Biol Chem. 1984 Jul 10;259(13):8246-51.