Lipskaia T Iu, Templ V D, Belousova L V, Molokova E V
Biokhimiia. 1980 Aug;45(8):1347-51.
The conditions for chromatographic separation in Silufol plates of adenine nucleotides, creatinine phosphate, glucose-6-phosphate and Pi have been found. Using this method, it was shown that in the presence of Pi and non-labelled ATP the specific radioactivity of creatine phosphate formed by mitochondrial creatinine kinase via oxidative phosphorylation increases at the same rate as does the specific radioactivity of the surrounding solution of ATP. It is concluded that under the given experimental conditions the ATP formed via oxidative phosphorylation enters the enzyme active center only after it has passed into the solution rather than immediately from the adenine nucleotide carrier.
已找到在硅氟板上对腺嘌呤核苷酸、磷酸肌酸、6-磷酸葡萄糖和无机磷酸盐进行色谱分离的条件。使用该方法表明，在存在无机磷酸盐和未标记的三磷酸腺苷（ATP）的情况下，线粒体肌酸激酶通过氧化磷酸化形成的磷酸肌酸的比放射性与周围ATP溶液的比放射性以相同速率增加。得出的结论是，在给定的实验条件下，通过氧化磷酸化形成的ATP只有在进入溶液后才进入酶活性中心，而不是直接从腺嘌呤核苷酸载体进入。
