Bougerolle A M, Chabard J L, Bargnoux H, Petit J, Berger J A, Dordain G
J Chromatogr. 1985 Nov 29;345(1):59-66. doi: 10.1016/0378-4347(85)80135-3.
A capillary column gas chromatography--mass fragmentographic method for metapramine and its three major demethylated metabolites is described. Compounds are extracted from plasma using a double-extraction procedure and transformed into N-trifluoroacetyl derivatives. The detection is performed by monitoring specific ions for metapramine and for its metabolites with a mass detector. In spite of extensive metabolism in the liver and rapid elimination of metapramine, plasma concentrations of both metapramine and its metabolites can be simultaneously followed over 24 h after a single 150-mg oral dose, because of the sensitivity and selectivity of the method. This method has been successfully applied to the analysis of samples obtained from patients who were at steady state with metapramine and to a pharmacokinetic study in a healthy volunteer.
本文描述了一种用于测定甲麦角林及其三种主要去甲基代谢物的毛细管柱气相色谱-质谱碎片分析法。采用双萃取程序从血浆中提取化合物，并将其转化为N-三氟乙酰基衍生物。通过用质量检测器监测甲麦角林及其代谢物的特定离子来进行检测。尽管甲麦角林在肝脏中广泛代谢且消除迅速，但由于该方法的灵敏度和选择性，单次口服150毫克剂量后，在24小时内可同时跟踪甲麦角林及其代谢物的血浆浓度。该方法已成功应用于从甲麦角林稳态患者获取的样本分析以及健康志愿者的药代动力学研究。
