Alfredsson G, Wiesel F A, Fyro B, Sedvall G
Psychopharmacology (Berl). 1977 Mar 23;52(1):25-30. doi: 10.1007/BF00426595.
A mass fragmentographic method for the quantification of clomipramine (CIM) and monodemethyl-clomipramine (DCIM) in human plasma was developed. The deuterium labelled analogues of the compounds were used as internal standards. The sensitivity of the method allows the determination of CIM and DCIM in plasma after oral doses with a standard deviation less than 7% at concentrations of 25 ng/ml. The method was applied to the analysis of drug concentrations in plasma of clomipramine treated healthy volunteers and depressed patients. After acute treatment the level of DCIM in plasma was low as compared to chronical treatment.
建立了一种用于定量人血浆中氯米帕明（CIM）和去甲基氯米帕明（DCIM）的质量碎片分析法。这些化合物的氘代类似物用作内标。该方法的灵敏度使得在口服剂量后，血浆中CIM和DCIM的浓度在25 ng/ml时，测定的标准偏差小于7%。该方法应用于分析氯米帕明治疗的健康志愿者和抑郁症患者血浆中的药物浓度。急性治疗后，与长期治疗相比，血浆中DCIM的水平较低。
