Bień Damian, Lange Agata, Matuszewski Arkadiusz, Ostrowska Agnieszka, Klimek Martyna, Batorska Martyna, Jaworski Sławomir
Department of Animal Breeding and Nutrition, Institute of Animal Sciences, Warsaw University of Life Sciences, 8 Ciszewskiego St, 02-786, Warsaw, Poland.
Department of Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences, 8 Ciszewskiego St, 02-786, Warsaw, Poland.
Sci Rep. 2025 Aug 27;15(1):31532. doi: 10.1038/s41598-025-17387-2.
Hydroxyapatite (HaP) is a biomaterial valued for its biocompatibility and osteoconductive properties, making it suitable for implantology and tissue engineering. The natural flavonoid quercetin (Q) exhibits potent antioxidant and anti-inflammatory effects that support wound healing and reduce oxidative stress. This study assessed the combined impact of HaP and Q on fibroblast viability, oxidative stress markers, and cell migration. The in ovo model evaluated systemic effects via morphometric parameters and oxidative stress in embryonic liver tissue.Results show that Q significantly decreased oxidative stress by increasing antioxidant enzyme activity and reducing intracellular ROS levels in fibroblasts and embryonic liver (p < 0.05). HaP alone induced a mild pro-oxidant response, but its combination with Q (QHaP) led to a marked reduction in oxidative stress markers, indicating a protective synergistic effect. Scratch assay revealed that Q and QHaP enhanced fibroblast migration at all concentrations, with HaP showing the highest wound closure (93.8%) and QHaP reaching 89.9% (p < 0.05). The in ovo model confirmed that QHaP-treated embryos developed normally, while Q improved embryonic antioxidant defenses. These findings suggest that QHaP composites are promising biocompatible materials that reduce oxidative stress and promote fibroblast migration for wound healing and tissue regeneration.
羟基磷灰石（HaP）是一种因其生物相容性和骨传导特性而受到重视的生物材料，使其适用于植入学和组织工程。天然类黄酮槲皮素（Q）具有强大的抗氧化和抗炎作用，有助于伤口愈合并降低氧化应激。本研究评估了HaP和Q对成纤维细胞活力、氧化应激标志物和细胞迁移的综合影响。鸡胚模型通过形态计量学参数和胚胎肝组织中的氧化应激评估全身效应。结果表明，Q通过增加成纤维细胞和胚胎肝中的抗氧化酶活性并降低细胞内ROS水平，显著降低了氧化应激（p < 0.05）。单独的HaP诱导了轻度的促氧化反应，但其与Q的组合（QHaP）导致氧化应激标志物显著降低，表明具有保护协同效应。划痕试验显示，Q和QHaP在所有浓度下均增强了成纤维细胞迁移，其中HaP显示出最高的伤口闭合率（93.8%），QHaP达到89.9%（p < 0.05）。鸡胚模型证实，经QHaP处理的胚胎发育正常，而Q改善了胚胎的抗氧化防御。这些发现表明，QHaP复合材料是有前景的生物相容性材料，可降低氧化应激并促进成纤维细胞迁移以促进伤口愈合和组织再生。
